{"id":"W2030519994","doi":"10.1016/j.pep.2010.05.005","title":"Production and purification of the penicillin-binding protein 3 from Pseudomonas aeruginosa","year":2010,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research","keywords":"Peptidoglycan; Penicillin binding proteins; Biochemistry; Pseudomonas aeruginosa; Escherichia coli; Glycan; Lipid II; Biology; Bacterial cell structure; Bacterial outer membrane; Cell wall; Microbiology; Bacteria; Chemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002012886,0.000119394,0.00009859001,0.00003513434,0.0001434893,0.00002795783,0.0001561258,0.000291709,0.00001245062],"category_scores_gemma":[0.0001538358,0.00008706722,0.00002898391,0.00007508227,0.0001739996,0.000008217528,0.0001004142,0.0001702726,0.000001577866],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003155477,"about_ca_system_score_gemma":0.00002864623,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002442745,"about_ca_topic_score_gemma":0.00001890001,"domain_scores_codex":[0.9991063,0.00006842821,0.0002198598,0.0003848355,0.0001075492,0.0001130429],"domain_scores_gemma":[0.9991907,0.000004217077,0.0002207073,0.0004607178,0.00008296502,0.00004072538],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005152303,0.00003785311,0.0005757256,0.00001888039,0.000005334094,3.58037e-8,0.00004392594,2.269518e-7,0.9900852,0.0002559598,0.00003754051,0.008887738],"study_design_scores_gemma":[0.0001755914,0.00005059769,0.004402689,0.00004815709,0.000008362058,0.00000326134,0.00003683349,0.00001897041,0.9881946,0.0004190361,0.006532587,0.0001093467],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9971539,0.0002522217,0.0001805993,0.001285451,0.0001756969,0.0008691759,0.00002022678,0.0000133964,0.00004938031],"genre_scores_gemma":[0.9951311,0.0001180501,0.003982409,0.00001128481,0.000174502,0.0001340493,0.00005973021,0.00001217821,0.0003766984],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00877839,"threshold_uncertainty_score":0.3550498,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009351723951580229,"score_gpt":0.2227170457631427,"score_spread":0.2133653218115624,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}