{"id":"W2031595735","doi":"10.3791/4041","title":"A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells","year":2012,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; University of Toronto","keywords":"Chinese hamster ovary cell; HEK 293 cells; Biology; Recombinant DNA; Cell culture; Pichia pastoris; Cell biology; Protein biosynthesis; Yeast; Embryonic stem cell; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001879523,0.000108002,0.0001568572,0.00003173698,0.0001261717,0.00001517072,0.00009421806,0.00007501077,0.00002840233],"category_scores_gemma":[0.00004754297,0.00006887922,0.0001198257,0.00002804212,0.00005252525,0.00001749727,0.0000621291,0.00005205784,2.900543e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001219915,"about_ca_system_score_gemma":0.000024241,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002009967,"about_ca_topic_score_gemma":5.14264e-7,"domain_scores_codex":[0.9992078,0.00004557746,0.0003340121,0.00011154,0.0001575588,0.0001435028],"domain_scores_gemma":[0.9992079,0.00001433608,0.0004154914,0.000149196,0.0001185091,0.00009453059],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005086064,0.0002164098,0.0007327794,0.00001459719,0.00009324837,1.734827e-7,0.0004264773,0.00001163348,0.9935002,0.00001842336,0.004312051,0.0001654531],"study_design_scores_gemma":[0.001148361,0.0005776105,0.0006006517,0.00006075554,0.00003778068,0.000006207417,0.000257875,0.00001830217,0.9906879,0.00004959307,0.00647172,0.00008323041],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.993975,0.003474111,0.001392781,0.00001583283,0.0007496871,0.000358535,0.00001376952,0.000002479565,0.00001781666],"genre_scores_gemma":[0.9974828,0.0001353794,0.00134239,0.00006270014,0.0007931626,0.00004169779,0.00001529688,0.00001459327,0.0001119179],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003507874,"threshold_uncertainty_score":0.2808813,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02801593237725729,"score_gpt":0.386515346195326,"score_spread":0.3584994138180687,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}