{"id":"W2032284140","doi":"10.1002/1526-968x(200011/12)28:3/4<147::aid-gene90>3.0.co;2-g","title":"Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon cre-mediated excision","year":2000,"lang":"en","type":"article","venue":"genesis","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":832,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lunenfeld-Tanenbaum Research Institute; University of Toronto; Mount Sinai Hospital; Sunnybrook Health Science Centre","funders":"Medical Research Council; Cancer Care Ontario","keywords":"Cre recombinase; Green fluorescent protein; Biology; Transgene; Molecular biology; Recombinase; lac operon; Embryonic stem cell; Reporter gene; Cre-Lox recombination; Genetically modified mouse; Gene; Cell culture; Gene knockin; Cell biology; Genetics; Gene expression","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003595852,0.0008871986,0.0006148384,0.001326843,0.0003982116,0.0005181175,0.001136102,0.0007864014,0.005026594],"category_scores_gemma":[0.0001523424,0.0003481084,0.000439473,0.0005510093,0.0004475658,0.0006015142,0.0005854995,0.001054669,0.004581512],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002472322,"about_ca_system_score_gemma":0.0004030594,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006865051,"about_ca_topic_score_gemma":0.001322375,"domain_scores_codex":[0.9997138,0.00002254354,0.00003121997,0.00008083322,0.0001056204,0.00004592667],"domain_scores_gemma":[0.9998454,0.00001320151,0.00005314728,0.00001914494,0.00002125491,0.00004790836],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001251395,0.00003839494,0.0001346061,0.00006740277,0.000008933687,0.0001365597,0.00001613705,0.00003614515,0.9925933,0.0004802328,0.001000618,0.005362401],"study_design_scores_gemma":[0.00008472355,0.0003081991,0.002533349,0.00002515262,0.00006581459,0.002382644,0.00003079745,0.0005402242,0.9312033,0.0002866366,0.06251542,0.00002369357],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5078004,0.01164037,0.3921025,0.001544652,0.001034802,0.001655097,0.02470635,0.01412932,0.04538646],"genre_scores_gemma":[0.553928,0.01660879,0.2202894,0.0007250714,0.000187513,0.001217847,0.0393969,0.002173843,0.1654726],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.005026594,"threshold_uncertainty_score":0.01681566,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01192882819464259,"score_gpt":0.2719604343695559,"score_spread":0.2600316061749133,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}