{"id":"W2034770360","doi":"10.1021/ac0004198","title":"Expression Hybridization Assays Combining cDNAs from Firefly and <i>Renilla</i> Luciferases as Labels for Simultaneous Determination of Two Target Sequences","year":2000,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Windsor","funders":"Universität Mannheim","keywords":"Chemistry; Luciferase; Luciferases; Biotinylation; Complementary DNA; Molecular biology; Bioluminescence; DNA; Hybridization probe; Molecular beacon; Primer (cosmetics); Biochemistry; Gene; Oligonucleotide; Biology; Transfection","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001698741,0.002073414,0.00152165,0.000944104,0.0006213569,0.0008264679,0.001207186,0.0008318654,0.003527214],"category_scores_gemma":[0.001019779,0.001074798,0.001456019,0.0009259331,0.001004878,0.0008636885,0.0009622478,0.002708578,0.002548219],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009433667,"about_ca_system_score_gemma":0.0006915181,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005535542,"about_ca_topic_score_gemma":0.001068177,"domain_scores_codex":[0.9979641,0.0004655943,0.0001857581,0.000582103,0.0004770073,0.0003254725],"domain_scores_gemma":[0.9993406,0.0002885844,0.00007459359,0.00007292904,0.0001346216,0.0000886749],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004861336,0.0000428995,0.00007972966,0.00007985339,0.000009147324,0.00001782783,0.00003106499,0.00005427252,0.9985589,0.0001700228,0.00007729521,0.0008303427],"study_design_scores_gemma":[0.00003057879,0.0002193169,0.0004746009,0.00001065567,0.00004643698,0.0001179068,0.00002667127,0.00111811,0.9944772,0.0001105773,0.003352152,0.00001585696],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4186231,0.003168953,0.5628894,0.0007176708,0.0004597004,0.001438743,0.00208364,0.002460142,0.008158618],"genre_scores_gemma":[0.3981809,0.0052418,0.5505176,0.0005976614,0.0002351821,0.004579965,0.0143224,0.0007530378,0.02557157],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003527214,"threshold_uncertainty_score":0.01179975,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01116314783194686,"score_gpt":0.2961953962569167,"score_spread":0.2850322484249698,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}