{"id":"W2034838611","doi":"10.1016/j.pep.2006.06.020","title":"High level production of the Magnaporthe grisea fructose 1,6-bisphosphate aldolase enzyme in Escherichia coli using a small volume bench-top fermentor","year":2006,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Magnaporthe grisea; Aldolase A; Escherichia coli; Fermentation; Enzyme; Biochemistry; Enzyme kinetics; Fructose; Enzyme assay; Biology; Chemistry; Fructose-bisphosphate aldolase; Active site; Oryza sativa","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003690014,0.0006727253,0.0006720008,0.0002816396,0.0002418694,0.0006092042,0.0004805003,0.0004284267,0.0006039085],"category_scores_gemma":[0.0002754855,0.0002426769,0.0004165765,0.0004481609,0.0002615137,0.000346552,0.0003975116,0.001114811,0.0007975898],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006583265,"about_ca_system_score_gemma":0.0003807716,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00240872,"about_ca_topic_score_gemma":0.002215746,"domain_scores_codex":[0.9997193,0.00004140779,0.00002946221,0.00004226075,0.0001208489,0.00004673054],"domain_scores_gemma":[0.9998567,0.00004337373,0.00002018848,0.00002502888,0.00002158112,0.00003317704],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006541438,0.0000560665,0.0001728725,0.00006101158,0.00001111521,0.00005081327,0.00002273808,0.0001616174,0.9973266,0.0001447066,0.0001098996,0.001817164],"study_design_scores_gemma":[0.00001407596,0.00007760502,0.001402628,0.000005535678,0.00001737463,0.00008728671,0.00001709528,0.0009711828,0.9951531,0.00006075409,0.002185671,0.000007669273],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9459943,0.001630849,0.04348496,0.0008370072,0.0002004397,0.0001605028,0.004284874,0.001004292,0.00240283],"genre_scores_gemma":[0.9318062,0.001873378,0.04688188,0.0001702582,0.00004180578,0.00008657666,0.008440807,0.0002075977,0.0104915],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00240872,"threshold_uncertainty_score":0.004789472,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01864887301089208,"score_gpt":0.2199851376042238,"score_spread":0.2013362645933318,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}