{"id":"W2035533607","doi":"10.1016/j.pep.2008.04.007","title":"A new protocol for high-yield purification of recombinant human CXCL8(3–72)K11R/G31P expressed in Escherichia coli","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Chemokine receptors and signaling","field":"Medicine","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"Royal University Hospital; University of Saskatchewan","funders":"National Tsing Hua University; National Science Council","keywords":"Interleukin 8; Escherichia coli; Recombinant DNA; Circular dichroism; Chemistry; Isothermal titration calorimetry; Yield (engineering); Chemokine; CXC chemokine receptors; Molecular biology; Biochemistry; Chromatography; Receptor; Biology; Inflammation; Materials science; Chemokine receptor; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003492179,0.0001910394,0.0003674445,0.0001624229,0.0001602631,0.00001683673,0.0001068481,0.0002019698,0.0001649291],"category_scores_gemma":[0.0001793393,0.00016181,0.00006905629,0.000224131,0.00005213692,0.0001368145,0.00002789782,0.0001851917,0.000002649796],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004372806,"about_ca_system_score_gemma":0.0001359703,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001690191,"about_ca_topic_score_gemma":0.000003503887,"domain_scores_codex":[0.9984106,0.00006232324,0.0006298494,0.0004107182,0.0002554424,0.0002310385],"domain_scores_gemma":[0.9989153,0.00005547544,0.0003671102,0.0003617563,0.0001539551,0.0001463896],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006056389,0.000349368,0.0009909931,0.000391764,0.000006640431,0.000001032868,0.0005685138,0.000002239415,0.9884264,0.0006381648,0.002161114,0.00585811],"study_design_scores_gemma":[0.00415865,0.0003366721,0.00703869,0.001032471,0.00001266189,0.000003493327,0.00007528446,0.000162497,0.9754767,0.001015353,0.01050685,0.0001806593],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.880707,0.00008677855,0.009660101,0.00103547,0.00006458828,0.1080001,0.000006220737,0.00007987535,0.0003598786],"genre_scores_gemma":[0.8626276,0.0000199369,0.02243901,0.00003440622,0.0001486334,0.1124938,0.00006846473,0.00003590942,0.002132289],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01807943,"threshold_uncertainty_score":0.659842,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04914976362170152,"score_gpt":0.3094128512875053,"score_spread":0.2602630876658038,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}