{"id":"W2035533607","doi":"10.1016/j.pep.2008.04.007","title":"A new protocol for high-yield purification of recombinant human CXCL8(3–72)K11R/G31P expressed in Escherichia coli","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Chemokine receptors and signaling","field":"Medicine","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"Royal University Hospital; University of Saskatchewan","funders":"National Tsing Hua University; National Science Council","keywords":"Interleukin 8; Escherichia coli; Recombinant DNA; Circular dichroism; Chemistry; Isothermal titration calorimetry; Yield (engineering); Chemokine; CXC chemokine receptors; Molecular biology; Biochemistry; Chromatography; Receptor; Biology; Inflammation; Materials science; Chemokine receptor; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007386626,0.001900878,0.001461896,0.001021555,0.0008718262,0.0006528641,0.001488077,0.000667248,0.003582947],"category_scores_gemma":[0.0005920028,0.000798722,0.0008841863,0.001370632,0.0003800974,0.0005738348,0.0007441601,0.002837934,0.005017649],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007816591,"about_ca_system_score_gemma":0.0008786588,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001907167,"about_ca_topic_score_gemma":0.002960511,"domain_scores_codex":[0.999166,0.0001482884,0.0001311579,0.0001328855,0.0002879007,0.0001337757],"domain_scores_gemma":[0.9997181,0.00006091585,0.00003692866,0.00007151684,0.00006608829,0.00004636599],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001676256,0.0001707774,0.0001721486,0.0003245762,0.00004375586,0.0002349462,0.0001109159,0.0003069385,0.9812452,0.0008021324,0.00363519,0.01278581],"study_design_scores_gemma":[0.000169474,0.0003134071,0.002203736,0.00005267101,0.000105884,0.0009645231,0.00004703975,0.002550069,0.9306555,0.000651463,0.06219164,0.00009446874],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.2037132,0.006028117,0.7582625,0.002101068,0.00106625,0.004614721,0.01168252,0.004145594,0.00838615],"genre_scores_gemma":[0.2198778,0.008991882,0.65846,0.0009990665,0.000306948,0.009384985,0.05787078,0.0009049054,0.04320362],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.003582947,"threshold_uncertainty_score":0.0119862,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04914976362170152,"score_gpt":0.3094128512875053,"score_spread":0.2602630876658038,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}