{"id":"W2036080216","doi":"10.1038/srep00246","title":"Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification","year":2012,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":220,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Multiple displacement amplification; Primer (cosmetics); DNA polymerase; DNA; Amplicon; Molecular biology; Loop-mediated isothermal amplification; DNA clamp; Denaturation (fissile materials); Biophysics; Polymerase chain reaction; Chemistry; Biology; Biochemistry; Reverse transcriptase; DNA extraction; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008505355,0.0001019776,0.00008781203,0.00005461705,0.0003185961,0.0001384136,0.0000541236,0.0001015114,0.000003842414],"category_scores_gemma":[0.0001222066,0.00009311853,0.00005483372,0.0001099113,0.0001353057,0.00001940499,0.00003917215,0.00002668631,5.600471e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001331893,"about_ca_system_score_gemma":0.00002528755,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000001411779,"about_ca_topic_score_gemma":0.000001138594,"domain_scores_codex":[0.9989331,0.00001961385,0.000257858,0.0004533632,0.0001236489,0.0002124055],"domain_scores_gemma":[0.9990377,0.000006677071,0.000228584,0.0004581519,0.0001949829,0.00007384062],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001109646,0.00003688188,0.001213088,0.00001327692,0.00001260195,3.52724e-7,0.00004237807,0.00009449082,0.9949738,0.0003791582,0.0003414147,0.002881466],"study_design_scores_gemma":[0.00006725656,0.00002601475,0.0004399959,0.000007254985,0.00002376149,0.00005890442,0.0000343262,0.0007910901,0.9791708,0.001171812,0.01805303,0.0001557266],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5573093,0.0001530214,0.4414155,0.0000583419,0.0006340511,0.0002843156,0.000004730411,0.00004419207,0.00009657911],"genre_scores_gemma":[0.9326242,0.00001524299,0.06574745,0.00003931626,0.0001753735,0.00003198075,0.0003854028,0.00001430084,0.0009667798],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3756681,"threshold_uncertainty_score":0.3797264,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01749872163086868,"score_gpt":0.2981365385458772,"score_spread":0.2806378169150086,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}