{"id":"W2036892804","doi":"10.1016/j.pep.2009.02.013","title":"Expression and purification of recombinant M-Pol I from Saccharomyces cerevisiae with α-1,6 mannosylpolymerase activity","year":2009,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University Health Centre; McGill University","funders":"Canadian Institutes of Health Research; National Institutes of Health; National Institute of General Medical Sciences; Canada Research Chairs","keywords":"Mannan; Mannose; Saccharomyces cerevisiae; Pichia pastoris; Recombinant DNA; Biochemistry; Glycan; Biology; Affinity chromatography; Glycoprotein; Yeast; Molecular biology; Enzyme; Polysaccharide; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002163517,0.0001864164,0.000184947,0.00008915067,0.0001648746,0.00005487633,0.0001352892,0.0001799887,0.00004653466],"category_scores_gemma":[0.00008002255,0.0001445753,0.00002960091,0.0001303772,0.00008895236,0.00003793802,0.00004890744,0.0001302278,0.000003104392],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008423174,"about_ca_system_score_gemma":0.00004412668,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005185221,"about_ca_topic_score_gemma":0.000004282012,"domain_scores_codex":[0.9986243,0.000173094,0.0002470892,0.0005187035,0.0002535403,0.0001833282],"domain_scores_gemma":[0.9990118,0.00001618444,0.0002421591,0.0004518293,0.0001414682,0.0001365956],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005601196,0.0001445579,0.0004669029,0.00003934215,0.000006630774,6.430527e-7,0.00009092555,0.000001415732,0.932263,0.00009753335,0.0001581963,0.06617072],"study_design_scores_gemma":[0.0008722643,0.0003876946,0.02273577,0.0002056001,0.000007912879,0.000003666515,0.00006619317,0.000116006,0.9710774,0.0002649769,0.004078929,0.000183532],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9924418,0.001957834,0.003558135,0.0008051094,0.00002443209,0.0009089179,0.00002031936,0.0000210741,0.0002623291],"genre_scores_gemma":[0.9948302,0.000645169,0.003664814,0.00003507134,0.00006014858,0.0001174804,0.0001635821,0.00001491981,0.0004686119],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06598719,"threshold_uncertainty_score":0.589561,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01312494766609399,"score_gpt":0.2630614379503641,"score_spread":0.2499364902842701,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}