{"id":"W2037363477","doi":"10.1021/bi9021268","title":"Switching Catalysis from Hydrolysis to Perhydrolysis in <i>Pseudomonas fluorescens</i> Esterase<sup>,</sup>","year":2010,"lang":"en","type":"article","venue":"Biochemistry","topic":"Protein Structure and Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":54,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute; McGill University","funders":"National Institute of General Medical Sciences; National Institute of Biomedical Imaging and Bioengineering; University of Minnesota","keywords":"Chemistry; Pseudomonas fluorescens; Hydrolysis; Hydrogen peroxide; Esterase; Active site; Stereochemistry; Acetic acid; Catalysis; Reaction rate constant; Peracetic acid; Serine; Medicinal chemistry; Enzyme; Organic chemistry; Kinetics; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001871174,0.000399726,0.000370574,0.0001000061,0.0001069224,0.00008110361,0.0006111173,0.000530338,0.00007059368],"category_scores_gemma":[0.0002272089,0.0004082141,0.0002504084,0.0003386576,0.00006356195,0.00000915527,0.0003316181,0.0004280618,0.00003425083],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003537851,"about_ca_system_score_gemma":0.0001090395,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004742117,"about_ca_topic_score_gemma":0.0002907732,"domain_scores_codex":[0.9977909,0.00003342766,0.0004279877,0.0009693169,0.0002781553,0.0005001896],"domain_scores_gemma":[0.9982589,0.00002491189,0.0001063179,0.001241332,0.00007009636,0.0002983667],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008670649,0.0000670601,0.008043154,0.00001711089,0.00009216462,0.00002373552,0.0001405368,0.0004829924,0.9873499,0.000003147739,0.00034479,0.003348701],"study_design_scores_gemma":[0.0005858623,0.00003543008,0.000832783,0.00002256363,0.00007287514,0.00002434519,0.00009369516,0.002354839,0.992656,0.00009812314,0.002671944,0.0005515541],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9977443,0.0002735842,0.0006153164,0.0002772278,0.00009576345,0.0001809655,0.0001128354,0.00003719539,0.0006627823],"genre_scores_gemma":[0.9929953,0.000025577,0.004285258,0.0006581387,0.0006908613,0.00006195458,0.0009081708,0.00005385607,0.0003209203],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007210371,"threshold_uncertainty_score":0.999837,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00279847478189188,"score_gpt":0.2100848562860596,"score_spread":0.2072863815041678,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}