{"id":"W2037479006","doi":"10.1016/j.chembiol.2012.09.016","title":"Active Site Plasticity within the Glycoside Hydrolase NagZ Underlies a Dynamic Mechanism of Substrate Distortion","year":2012,"lang":"en","type":"article","venue":"Chemistry & Biology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":82,"is_retracted":false,"has_abstract":false,"ca_institutions":"Simon Fraser University; University of Manitoba","funders":"Canadian Institutes of Health Research","keywords":"Hydrolase; Active site; Glycoside hydrolase; Peptidoglycan; Bacillus subtilis; Substrate (aquarium); Bacterial cell structure; Biochemistry; Enzyme; Biology; Histidine; Stereochemistry; Bacteria; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002703588,0.0002542582,0.0004062724,0.0002450546,0.0003023608,0.001167309,0.0007447319,0.0005320531,0.001383461],"category_scores_gemma":[0.0008585087,0.0004037694,0.0003149438,0.0001848642,0.0007531245,0.001084683,0.0007760662,0.00083224,0.0005133932],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003072025,"about_ca_system_score_gemma":0.0001737601,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002411691,"about_ca_topic_score_gemma":0.0003126933,"domain_scores_codex":[0.9997982,0.0000167849,0.00001360267,0.00005722203,0.00007247616,0.00004166403],"domain_scores_gemma":[0.9996533,0.00009975237,0.00007663291,0.00008938701,0.00003218832,0.00004871799],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001657384,0.00003394819,0.002437005,0.0000269935,0.00001810525,0.0002546292,0.00008947502,0.002025743,0.9836718,0.003144767,0.00008147306,0.008050395],"study_design_scores_gemma":[0.00007260788,0.0004067617,0.04335065,0.00001379418,0.00006251554,0.003068339,0.0003659761,0.076512,0.8620033,0.01135369,0.0026777,0.0001126779],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9901978,0.0001860573,0.008447708,0.0001117347,0.00001510968,0.000007262505,0.00003619504,0.0001374545,0.0008606449],"genre_scores_gemma":[0.9986392,0.00007546675,0.0009825467,0.00001686382,0.000005698732,0.000003418608,0.00003844438,0.00001958057,0.0002188563],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001383461,"threshold_uncertainty_score":0.004628122,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00742721837608312,"score_gpt":0.2321516012912106,"score_spread":0.2247243829151275,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}