{"id":"W2037479006","doi":"10.1016/j.chembiol.2012.09.016","title":"Active Site Plasticity within the Glycoside Hydrolase NagZ Underlies a Dynamic Mechanism of Substrate Distortion","year":2012,"lang":"en","type":"article","venue":"Chemistry & Biology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":82,"is_retracted":false,"has_abstract":false,"ca_institutions":"Simon Fraser University; University of Manitoba","funders":"Canadian Institutes of Health Research","keywords":"Hydrolase; Active site; Glycoside hydrolase; Peptidoglycan; Bacillus subtilis; Substrate (aquarium); Bacterial cell structure; Biochemistry; Enzyme; Biology; Histidine; Stereochemistry; Bacteria; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001197033,0.0001467147,0.000131592,0.00001217089,0.00008373362,0.00000624392,0.0001230603,0.0002016365,0.00003535572],"category_scores_gemma":[0.0001021945,0.0001145337,0.00006392435,0.00006501218,0.0001802656,0.000008124006,0.00006408726,0.0001165534,0.000007711033],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002638421,"about_ca_system_score_gemma":0.00003992919,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008226377,"about_ca_topic_score_gemma":0.000009843484,"domain_scores_codex":[0.9992309,0.00005248957,0.0002183868,0.0002417081,0.00005562067,0.0002009181],"domain_scores_gemma":[0.9994029,0.000013963,0.0002308157,0.0002259778,0.00006695865,0.00005935006],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001226585,0.00006490821,0.001154454,0.00002711628,0.00004105255,1.678467e-7,0.0001229272,0.00001225219,0.9980724,0.0002103173,0.0000282277,0.0001435015],"study_design_scores_gemma":[0.0002073246,0.00005270432,0.002607219,0.000005958922,0.00002900033,0.00002507321,0.0001276185,0.00009948168,0.9956016,0.0002314775,0.0008853382,0.0001272018],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9955732,0.0001734597,0.003420446,0.0001421165,0.0002676753,0.00009472168,0.00005452268,0.00001653366,0.0002573105],"genre_scores_gemma":[0.9985341,0.00005993841,0.00005643821,0.00007900936,0.0002556878,0.0000213594,0.0006433693,0.00001361172,0.0003364935],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003364008,"threshold_uncertainty_score":0.4670549,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00742721837608312,"score_gpt":0.2321516012912106,"score_spread":0.2247243829151275,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}