{"id":"W2038036381","doi":"10.1139/w00-007","title":"Overproduction in <i>Escherichia coli </i>of the pectin methylesterase A from <i>Erwinia chrysanthemi</i> 3937: One-step purification, biochemical characterization, and production of polyclonal antibodies","year":2000,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Polysaccharides and Plant Cell Walls","field":"Agricultural and Biological Sciences","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institut National de la Recherche Agronomique","keywords":"Polyclonal antibodies; Pectin; Escherichia coli; Pectinase; Pectinesterase; Divalent; Erwinia; Chemistry; Biochemistry; Affinity chromatography; Pathogen; Pectate lyase; Enzyme; Biology; Microbiology; Antibody; Organic chemistry","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002320174,0.0008057772,0.000419272,0.0002253946,0.0001429572,0.0003440779,0.0004011591,0.0003226256,0.0008126852],"category_scores_gemma":[0.0001939032,0.0002394763,0.000323753,0.0005887146,0.0002062547,0.0001881012,0.0003385914,0.0005089073,0.0006941946],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006845976,"about_ca_system_score_gemma":0.0002670371,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003092175,"about_ca_topic_score_gemma":0.001927959,"domain_scores_codex":[0.999678,0.00005301113,0.00004478628,0.00006596902,0.00009452131,0.00006365013],"domain_scores_gemma":[0.9998044,0.0000413399,0.00006062916,0.00003910312,0.00002142339,0.00003307403],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006556211,0.0000428053,0.0001379691,0.00004380142,0.000006042432,0.00004499688,0.00001577087,0.00006309595,0.9988359,0.00008047045,0.00004346093,0.0006200832],"study_design_scores_gemma":[0.00001962527,0.0001493434,0.002630743,0.000006851712,0.00002023217,0.0002324782,0.00002027845,0.0006590651,0.9934928,0.00003210977,0.002731287,0.000005063057],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9886567,0.0007071255,0.006207931,0.0001773085,0.00004992133,0.0001266936,0.002071912,0.0002493526,0.00175299],"genre_scores_gemma":[0.9762763,0.0007792969,0.01097658,0.00008840214,0.00001698637,0.0001082405,0.006433703,0.0001189535,0.00520151],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003092175,"threshold_uncertainty_score":0.006148338,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009046749481982426,"score_gpt":0.1784370325081182,"score_spread":0.1693902830261358,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}