{"id":"W2038059735","doi":"10.1016/s1046-5928(03)00010-x","title":"Expression of a Brassic napus glutamate 1-semialdehyde aminotransferase in Escherichia coli and characterization of the recombinant protein","year":2003,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Photosynthetic Processes and Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"Agriculture and Agri-Food Canada; Plant Biotechnology Institute","funders":"","keywords":"Recombinant DNA; Biochemistry; Escherichia coli; Biology; Enzyme; Complementary DNA; Affinity chromatography; Molecular biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002454703,0.0001159889,0.0001399819,0.00003552141,0.00006046077,0.00001079021,0.00009436742,0.0001376258,0.000007549816],"category_scores_gemma":[0.00009045561,0.00008422245,0.0000271188,0.0001018711,0.00006285478,0.00001264671,0.00003709735,0.00007309372,1.603258e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005128132,"about_ca_system_score_gemma":0.00005022784,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009565715,"about_ca_topic_score_gemma":0.000002226342,"domain_scores_codex":[0.999087,0.0001567552,0.0002792796,0.0002494319,0.0001111638,0.0001163415],"domain_scores_gemma":[0.9994571,0.00000509518,0.0002037372,0.0002384272,0.00005262746,0.00004299889],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001045907,0.0001034914,0.0001474501,0.0001642896,0.000001971478,1.302535e-7,0.0001471371,6.277858e-7,0.99784,0.000403524,0.000001139132,0.001085636],"study_design_scores_gemma":[0.0005625194,0.00009481474,0.001440475,0.0004054364,0.000004832743,0.000001891988,0.00004511454,0.00004081695,0.9960071,0.0005398318,0.0007576703,0.0000994433],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9956542,0.0001888204,0.003032715,0.00006949796,0.00004100049,0.0009426571,0.00001209644,0.000004638642,0.00005434076],"genre_scores_gemma":[0.998776,0.0002035324,0.0006032091,0.00001808302,0.000008262948,0.0002087641,0.00002071234,0.00001222623,0.0001491929],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003121784,"threshold_uncertainty_score":0.3434492,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007081852029036486,"score_gpt":0.2109720091282038,"score_spread":0.2038901570991673,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}