{"id":"W2038181471","doi":"10.1007/s00216-009-3150-9","title":"Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits","year":2009,"lang":"en","type":"review","venue":"Analytical and Bioanalytical Chemistry","topic":"Genetically Modified Organisms Research","field":"Agricultural and Biological Sciences","cited_by":406,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"","keywords":"Biotechnology; DNA extraction; Canola; Biochemical engineering; Traceability; Genetically modified organism; genomic DNA; Computational biology; Biology; Veterinary Drugs; Sample preparation; DNA; Computer science; Chromatography; Polymerase chain reaction; Chemistry; Food science; Genetics; Engineering; Gene; Medicine; Veterinary medicine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["research_integrity"],"consensus_categories":[],"category_scores_codex":[0.001690409,0.0005211555,0.001876332,0.00008625169,0.0000969418,0.00005366373,0.000453467,0.001377282,0.0001650563],"category_scores_gemma":[0.001670329,0.0002347511,0.0003992041,0.001057743,0.001094669,0.00005890799,0.0002005376,0.0007440644,0.00001061463],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005155949,"about_ca_system_score_gemma":0.00006634789,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004997962,"about_ca_topic_score_gemma":0.000002442843,"domain_scores_codex":[0.9962236,0.0005632142,0.001237445,0.001017609,0.0004768929,0.0004812127],"domain_scores_gemma":[0.9962322,0.002507064,0.0004573075,0.0002433136,0.0001920778,0.0003680776],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001625754,0.0001242083,0.000002099544,0.0008066545,0.00008001669,0.000002398825,0.000002027021,4.930706e-7,0.4451691,0.0002367395,0.00001331364,0.5535467],"study_design_scores_gemma":[0.0003809897,0.001221017,0.003839591,0.004122165,0.002906189,0.0001295749,0.00009235237,0.001603227,0.9166848,0.00252332,0.06482863,0.001668126],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.3559228,0.6342723,0.0005612569,0.003375335,0.00005203927,0.00195119,0.0004074571,0.0003000745,0.003157463],"genre_scores_gemma":[0.0232009,0.9684861,0.007732815,0.00002244845,0.0001259419,0.00001500129,0.0001075909,0.000009285711,0.0002999657],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.5518786,"threshold_uncertainty_score":0.9999191,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06198714182598661,"score_gpt":0.375502195847996,"score_spread":0.3135150540220094,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}