{"id":"W2038267920","doi":"10.1128/aem.70.10.6123-6130.2004","title":"Helix 4 Mutants of the <i>Bacillus thuringiensis</i> Insecticidal Toxin Cry1Aa Display Altered Pore-Forming Abilities","year":2004,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Mutant; Bacillus thuringiensis; Biophysics; Biochemistry; Helix (gastropod); Amino acid; Brush border; Mutagenesis; Chemistry; Vesicle; Biology; Membrane; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001320891,0.0004025165,0.0002256768,0.000151914,0.00009839705,0.0002315045,0.0001741929,0.0002757346,0.0005979385],"category_scores_gemma":[0.0001872681,0.0001392308,0.0003079101,0.0001265027,0.0002749573,0.0001444052,0.00017678,0.0004260663,0.0003613552],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002556983,"about_ca_system_score_gemma":0.0001245422,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004338874,"about_ca_topic_score_gemma":0.0005146195,"domain_scores_codex":[0.999872,0.0000368832,0.00001669268,0.0000193306,0.0000315377,0.0000235169],"domain_scores_gemma":[0.999741,0.00004681448,0.0001314299,0.00002419297,0.00001632228,0.0000401701],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009940404,0.00001560838,0.0002994031,0.00001254007,0.000007609214,0.00005794151,0.000007539005,0.000125791,0.9988804,0.00004713173,0.00001081329,0.0004357347],"study_design_scores_gemma":[0.0000396129,0.0003677405,0.007086448,0.000006839102,0.00003955917,0.00082821,0.0000288925,0.001436725,0.9891367,0.00006999741,0.0009484411,0.00001091496],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9986841,0.00009739839,0.0007788391,0.00001881598,0.000006601348,0.000007734136,0.0001059765,0.00002438299,0.0002761971],"genre_scores_gemma":[0.9975904,0.0001138659,0.001434051,0.0000262649,0.000005459807,0.000007412128,0.0003182573,0.00001915035,0.0004851237],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005979385,"threshold_uncertainty_score":0.002000272,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002946508092121315,"score_gpt":0.1671165658685949,"score_spread":0.1641700577764735,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}