{"id":"W2038384389","doi":"10.1021/bi9009049","title":"A Thioesterase from an Iterative Fungal Polyketide Synthase Shows Macrocyclization and Cross Coupling Activity and May Play a Role in Controlling Iterative Cycling through Product Offloading","year":2009,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":51,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"National Institute of General Medical Sciences","keywords":"Thioesterase; Polyketide synthase; ATP synthase; Chemistry; Polyketide; Product (mathematics); Coupling (piping); Biochemistry; Combinatorial chemistry; Enzyme; Biosynthesis; Materials science","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001637775,0.0004503825,0.0002122787,0.0001091357,0.00009932998,0.000303416,0.0001639128,0.0003213759,0.0007780145],"category_scores_gemma":[0.0002433366,0.0001262739,0.0002897136,0.0001629543,0.0001842474,0.000312092,0.0002053213,0.0004677657,0.0005267411],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001741404,"about_ca_system_score_gemma":0.0001869508,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003443875,"about_ca_topic_score_gemma":0.0005162479,"domain_scores_codex":[0.9999057,0.00001325054,0.00001011303,0.00002278767,0.00002880483,0.00001927867],"domain_scores_gemma":[0.9998677,0.00003092327,0.00003039668,0.00002445467,0.00002058641,0.00002586453],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002017216,0.00001033768,0.0001859635,0.00001016518,0.000002083858,0.000028496,0.000005681231,0.00004771672,0.9981201,0.00007003769,0.00000613458,0.00149296],"study_design_scores_gemma":[0.000002270885,0.00006245637,0.001665762,0.000001561889,0.000004390658,0.0002066894,0.00001182826,0.0004156155,0.9970399,0.00004560559,0.0005407824,0.00000320552],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9748327,0.0006777697,0.0224013,0.0001020235,0.00002546216,0.00003557316,0.0001798451,0.0001272917,0.001617962],"genre_scores_gemma":[0.9862071,0.0004374007,0.009658145,0.00002086487,0.000003808944,0.0000137385,0.0006413978,0.00002306997,0.002994547],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007780145,"threshold_uncertainty_score":0.002602756,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008959672268121771,"score_gpt":0.284802083877867,"score_spread":0.2758424116097452,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}