{"id":"W2038499837","doi":"10.1007/s10295-014-1437-0","title":"Functional expression of a single-chain antibody fragment against human epidermal growth factor receptor 2 (HER2) in<i>Escherichia coli</i>","year":2014,"lang":"en","type":"article","venue":"Journal of Industrial Microbiology & Biotechnology","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":34,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Isfahan University of Medical Sciences","keywords":"Receptor; Molecular biology; Single-chain variable fragment; Epidermal growth factor receptor; Biology; Escherichia coli; Tyrosine kinase; Monoclonal antibody; Epidermal growth factor; Signal transduction; Antibody; Affinity chromatography; Receptor tyrosine kinase; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000295223,0.0004977692,0.0003485112,0.0002160334,0.0001483327,0.0004477794,0.0003439572,0.0003291687,0.000593211],"category_scores_gemma":[0.000147787,0.0001505798,0.0003151,0.0004845806,0.0001776884,0.0001914202,0.0002470102,0.0005681943,0.0005800201],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004094084,"about_ca_system_score_gemma":0.0002436239,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008943314,"about_ca_topic_score_gemma":0.0007291985,"domain_scores_codex":[0.9997357,0.0000618784,0.00003460084,0.00004441616,0.00007980398,0.00004358068],"domain_scores_gemma":[0.9999174,0.00001506445,0.00002189578,0.00001271718,0.00001665259,0.00001616253],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005224013,0.000039448,0.0001319147,0.00004506139,0.000005210303,0.00005149398,0.000009024835,0.00006270754,0.9986522,0.0001055007,0.00007293616,0.0007722609],"study_design_scores_gemma":[0.00001037413,0.0001214086,0.001207353,0.000006175052,0.00001341875,0.0002021373,0.00001613862,0.0009686828,0.9937872,0.00002222604,0.003641174,0.000003702458],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9613796,0.002009655,0.02940155,0.0003279224,0.0001380732,0.0001738083,0.003094007,0.0002789127,0.003196439],"genre_scores_gemma":[0.9474152,0.001631945,0.03457087,0.0001348761,0.00002370648,0.0001006613,0.008245572,0.00009288733,0.007784202],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008943314,"threshold_uncertainty_score":0.002970517,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03907662077614878,"score_gpt":0.2844087282640531,"score_spread":0.2453321074879043,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}