{"id":"W2038825520","doi":"10.1006/plas.2001.1530","title":"Development of Small High-Copy-Number Plasmid Vectors for Gene Expression in Caulobacter crescentus","year":2001,"lang":"en","type":"article","venue":"Plasmid","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Caulobacter crescentus; Plasmid; Biology; Replicon; T-DNA Binary system; S-layer; Origin of replication; Escherichia coli; Gene; Expression vector; Shuttle vector; ColE1; Recombinant DNA; Plasmid preparation; Molecular biology; Genetics; PBR322; Vector (molecular biology); Cell cycle","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001035332,0.001155074,0.000914615,0.001118058,0.0006825559,0.0009179395,0.001252319,0.0007301759,0.001755334],"category_scores_gemma":[0.0009918115,0.0007638278,0.0008108914,0.001226659,0.000648779,0.0007901814,0.0005094004,0.002683264,0.001507307],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001254091,"about_ca_system_score_gemma":0.001423541,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003501041,"about_ca_topic_score_gemma":0.005751173,"domain_scores_codex":[0.99954,0.00005641107,0.00005594571,0.00008449556,0.0001965312,0.00006664234],"domain_scores_gemma":[0.998833,0.0003384526,0.000175878,0.0001681046,0.0002112505,0.0002733707],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001401913,0.0001070825,0.0001193908,0.00013392,0.00001285093,0.00005538882,0.00004407798,0.0001765943,0.9931471,0.00075933,0.000376403,0.004927605],"study_design_scores_gemma":[0.00005844888,0.0001332897,0.000976571,0.0000167637,0.00002852072,0.0001692938,0.00002116418,0.001811012,0.9898857,0.0001819909,0.006702696,0.00001456075],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5512888,0.003526659,0.4243216,0.001288848,0.0005610731,0.002122672,0.007381996,0.004486796,0.005021653],"genre_scores_gemma":[0.5737327,0.003008305,0.3752666,0.0003433356,0.00009929663,0.001475299,0.02661924,0.001483677,0.01797157],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003501041,"threshold_uncertainty_score":0.009099126,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01247327894165873,"score_gpt":0.2299939763595346,"score_spread":0.2175206974178759,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}