{"id":"W2038900290","doi":"10.1021/bi035296a","title":"Glutamate 346 of Human Na<sup>+</sup>−H<sup>+</sup> Exchanger NHE1 Is Crucial for Modulating both the Affinity for Na<sup>+</sup> and the Interaction with Amiloride Derivatives","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Ion Transport and Channel Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"","keywords":"Chemistry; Mutant; Amino acid; Wild type; Sodium–hydrogen antiporter; Methane sulfonate; Guanidine; Amiloride; Stereochemistry; Sodium; Mutation; Crystallography; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001382503,0.000416452,0.0004186332,0.0001072605,0.0001534692,0.0003140994,0.0003191777,0.000428774,0.001287117],"category_scores_gemma":[0.0002037047,0.0001959822,0.000358153,0.0001814082,0.0002923838,0.0001848598,0.0002508321,0.0003420234,0.0009683928],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002984062,"about_ca_system_score_gemma":0.000187391,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008007773,"about_ca_topic_score_gemma":0.0005751767,"domain_scores_codex":[0.9997759,0.00005709516,0.00003653365,0.00004306827,0.00005705989,0.00003018749],"domain_scores_gemma":[0.9998703,0.00002025088,0.00004323358,0.00001762638,0.00001372604,0.00003488996],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005334589,0.00002396129,0.00050715,0.00003803571,0.00001270247,0.0001830999,0.00001393407,0.0001594563,0.9973653,0.0001143336,0.00002947238,0.001018982],"study_design_scores_gemma":[0.00006991539,0.0005888356,0.008836439,0.00000763311,0.00004544702,0.001340284,0.00003405182,0.001028319,0.9841052,0.00005964062,0.003871539,0.0000127953],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9962908,0.0006509987,0.001778554,0.00008765259,0.0000271819,0.00003029837,0.0004899071,0.00003543002,0.0006091071],"genre_scores_gemma":[0.9945974,0.0003637048,0.002698405,0.00006081852,0.00001402224,0.00001676844,0.001085375,0.00002396622,0.00113951],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001287117,"threshold_uncertainty_score":0.00430578,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01700522382838207,"score_gpt":0.2548861201555418,"score_spread":0.2378808963271597,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}