{"id":"W2039213678","doi":"10.1016/s1570-9639(03)00105-5","title":"Soluble human core 2 β6-N-acetylglucosaminyltransferase C2GnT1 requires its conserved cysteine residues for full activity","year":2003,"lang":"en","type":"article","venue":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":false,"ca_institutions":"Toronto General Hospital; Hospital for Sick Children; Queen's University","funders":"Cystic Fibrosis Foundation","keywords":"Cysteine; Biochemistry; Enzyme; Dithiothreitol; Chemistry; Mutagenesis; Site-directed mutagenesis; Mutant; Active site; Serine; DTNB; Enzyme assay; Transferase; Glutathione","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002379479,0.0005198073,0.0004187482,0.0001859655,0.0001974104,0.0006059305,0.0002432741,0.0003791796,0.0009103222],"category_scores_gemma":[0.0002808762,0.0001720647,0.00030122,0.0003141988,0.0002215078,0.000252148,0.000212002,0.000417097,0.0008007712],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001069373,"about_ca_system_score_gemma":0.0006041431,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00288323,"about_ca_topic_score_gemma":0.003769256,"domain_scores_codex":[0.9998726,0.00001448249,0.0000103658,0.00003824174,0.00002858114,0.00003566434],"domain_scores_gemma":[0.9997793,0.00003379937,0.00003982984,0.00003274854,0.0000445274,0.00006985056],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002147674,0.00002458443,0.0004979882,0.00004114366,0.000009107882,0.00008835019,0.00002596886,0.0000598114,0.9976725,0.0001508195,0.0001501413,0.001064682],"study_design_scores_gemma":[0.00003582597,0.0001036347,0.008245029,0.000006531541,0.00001362467,0.0007856151,0.0000376258,0.001123835,0.9848289,0.0001040539,0.004705312,0.00001012467],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9935107,0.001948368,0.002390322,0.000154383,0.00006675637,0.00002921726,0.0008225662,0.0001117861,0.0009658253],"genre_scores_gemma":[0.9853756,0.0007232571,0.002243524,0.00008825337,0.00002330529,0.00003165568,0.006642042,0.0000592591,0.004813159],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00288323,"threshold_uncertainty_score":0.007758856,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04373559431130552,"score_gpt":0.3142948535348321,"score_spread":0.2705592592235266,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}