{"id":"W2039360539","doi":"10.1021/bi035396g","title":"Mutagenesis of the Conserved Active-Site Tyrosine Changes a Retaining Sialidase into an Inverting Sialidase","year":2003,"lang":"en","type":"article","venue":"Biochemistry","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":75,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"","keywords":"Enzyme kinetics; Sialidase; Chemistry; Mutant; Tyrosine; Stereochemistry; Glycosidic bond; Active site; Wild type; Saturated mutagenesis; Enzyme; Biochemistry; Cleavage (geology); Biology; Neuraminidase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001912569,0.0004887382,0.0002381234,0.0001494516,0.0001066304,0.0002723475,0.0003574762,0.000317104,0.0007110962],"category_scores_gemma":[0.0003226155,0.0001694174,0.0004042034,0.0002205287,0.0003223975,0.000128625,0.0002776265,0.0003904332,0.0004477458],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003635499,"about_ca_system_score_gemma":0.0004201024,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001960608,"about_ca_topic_score_gemma":0.001355701,"domain_scores_codex":[0.9998507,0.0000301012,0.00002784803,0.00002549722,0.00003720772,0.00002849731],"domain_scores_gemma":[0.9997583,0.00006325149,0.00006344083,0.00003284851,0.00002889396,0.00005336754],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001684681,0.00003806283,0.0005136597,0.00002215448,0.00002011001,0.0002411277,0.00003104329,0.0003788049,0.9968077,0.0002446928,0.0000252594,0.001508941],"study_design_scores_gemma":[0.00004193382,0.0004146078,0.003899104,0.0000105755,0.00005093152,0.000701023,0.00006405325,0.003402008,0.9897384,0.00009274863,0.001567438,0.00001718841],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9957587,0.0001000282,0.003342615,0.00003586567,0.00002209584,0.00003427888,0.0001831613,0.00005198372,0.0004712667],"genre_scores_gemma":[0.996245,0.000134306,0.002662145,0.00002410131,0.00000480671,0.00001115047,0.0002348592,0.00003656336,0.0006471236],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001960608,"threshold_uncertainty_score":0.003898442,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02069040182077415,"score_gpt":0.2356708644325542,"score_spread":0.2149804626117801,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}