{"id":"W2040273336","doi":"10.3791/50057","title":"Whole Mount RNA Fluorescent <em>in situ</em> Hybridization of <em>Drosophila</em> Embryos","year":2013,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; Montreal Clinical Research Institute","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"RNA; In situ hybridization; Biology; Locked nucleic acid; Oligonucleotide; Gene expression; Computational biology; Nucleic acid; Molecular biology; Gene; Cell biology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004392635,0.0009418117,0.0004880739,0.0008630736,0.0005633464,0.0005450457,0.0008541237,0.0007654874,0.01317536],"category_scores_gemma":[0.0002293114,0.0007134941,0.0006423401,0.0005657859,0.0005335764,0.0005936213,0.0004295357,0.001362228,0.01117348],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004001378,"about_ca_system_score_gemma":0.0004674414,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008500321,"about_ca_topic_score_gemma":0.003001572,"domain_scores_codex":[0.999645,0.00002821134,0.00003880965,0.000137518,0.0001044284,0.00004597574],"domain_scores_gemma":[0.9998255,0.00003483298,0.00003159434,0.00004418297,0.00004127797,0.00002270569],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001600059,0.000009185458,0.00005454765,0.0001149676,0.000004529628,0.00007800241,0.00001716559,0.00005932554,0.9942659,0.0003456505,0.0004298865,0.004604889],"study_design_scores_gemma":[0.0000152844,0.00009774131,0.003522479,0.00005989194,0.00003537131,0.0008480739,0.0000366825,0.001440993,0.9319794,0.0002513667,0.06169251,0.00002009491],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1246346,0.004947532,0.8062677,0.0005829176,0.0008747387,0.001603608,0.0121248,0.006358206,0.04260583],"genre_scores_gemma":[0.1319671,0.008633411,0.7660738,0.0006007816,0.0001515173,0.002346786,0.01768662,0.002939466,0.06960048],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01317536,"threshold_uncertainty_score":0.04407603,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01690648721583918,"score_gpt":0.3340853241199467,"score_spread":0.3171788369041075,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}