{"id":"W2041442194","doi":"10.1074/jbc.m407606200","title":"Site-saturation Mutagenesis of Tyr-105 Reveals Its Importance in Substrate Stabilization and Discrimination in TEM-1 β-Lactamase","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Antibiotic Resistance in Bacteria","field":"Biochemistry, Genetics and Molecular Biology","cited_by":60,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; Regroupement Québécois sur les Matériaux de Pointe","funders":"Natural Sciences and Engineering Research Council of Canada; McGill University","keywords":"Saturated mutagenesis; Active site; Enzyme kinetics; Stereochemistry; Escherichia coli; Chemistry; Mutant; Residue (chemistry); Site-directed mutagenesis; Mutagenesis; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001333605,0.0002953446,0.0001939835,0.00007636753,0.0001024543,0.0001812362,0.000201634,0.0002209644,0.0006463447],"category_scores_gemma":[0.0002728439,0.0001052624,0.0002478285,0.00009846989,0.0001779044,0.0001263592,0.0001576455,0.0003388253,0.0001636818],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002503662,"about_ca_system_score_gemma":0.0001635971,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007821394,"about_ca_topic_score_gemma":0.0008960821,"domain_scores_codex":[0.9999173,0.00002301053,0.00001108636,0.00001571506,0.00001730564,0.00001560574],"domain_scores_gemma":[0.9998423,0.00005665574,0.00005305667,0.00001066256,0.00001373178,0.00002361639],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003797352,0.0000817047,0.001933739,0.00003992582,0.0000212425,0.0001836139,0.00003573686,0.001410337,0.9934264,0.0002031539,0.00002908723,0.002255242],"study_design_scores_gemma":[0.0000652187,0.001336388,0.01555904,0.0000288482,0.00006233493,0.0009508044,0.00008947659,0.01445655,0.9661949,0.0001025447,0.001131578,0.00002236652],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9988332,0.0000788449,0.0008429672,0.00001226539,0.000003150676,0.000005511371,0.00002419441,0.000009095723,0.0001907311],"genre_scores_gemma":[0.9989268,0.00005974359,0.0007903457,0.00001548797,0.000001380314,0.000003790151,0.00005699724,0.000006589113,0.0001387237],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007821394,"threshold_uncertainty_score":0.002162278,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01754996977575852,"score_gpt":0.2613360247083202,"score_spread":0.2437860549325617,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}