{"id":"W2041540935","doi":"10.1007/s00253-013-5361-4","title":"Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly","year":2013,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":105,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Directorate for Biological Sciences; Petroleum Technology Research Centre; Lawrence Berkeley National Laboratory; Biological and Environmental Research; National Science Foundation","keywords":"Mevalonate pathway; Farnesyl pyrophosphate; Biochemistry; Biology; Gene; Metabolic pathway; Translational efficiency; Metabolic engineering; Operon; Isopentenyl pyrophosphate; Computational biology; Biosynthesis; Translation (biology); Escherichia coli; Messenger RNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004168536,0.001287123,0.001471969,0.0007960777,0.0004027428,0.001303103,0.0007710923,0.0005555426,0.001006384],"category_scores_gemma":[0.0007162461,0.0005588119,0.001026089,0.001488376,0.0004238157,0.0003574402,0.0007235321,0.00129064,0.0004524243],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008001385,"about_ca_system_score_gemma":0.0006550533,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009738936,"about_ca_topic_score_gemma":0.002507332,"domain_scores_codex":[0.9995642,0.00009213431,0.00004530136,0.00009248151,0.0001322529,0.00007358552],"domain_scores_gemma":[0.9997364,0.00007841745,0.00006612353,0.00004599328,0.00003971802,0.00003342545],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005041169,0.0004234949,0.0004420188,0.0001447345,0.00007082059,0.0001812046,0.00003263287,0.03482373,0.9412184,0.001818339,0.0001895351,0.02015091],"study_design_scores_gemma":[0.00008706652,0.0005022235,0.0003424533,0.00001044626,0.0001087752,0.0001453731,0.00003853383,0.07433587,0.9221492,0.000499655,0.001738952,0.00004137689],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9086706,0.0008479442,0.08544742,0.0002051043,0.000113127,0.0001814271,0.0003299488,0.001098916,0.003105542],"genre_scores_gemma":[0.9140261,0.0006590728,0.08286299,0.00005460303,0.00001894907,0.0001364013,0.0004444844,0.000290947,0.001506523],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001471969,"threshold_uncertainty_score":0.005805373,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005562153533055654,"score_gpt":0.1851450243881091,"score_spread":0.1795828708550535,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}