{"id":"W2041559837","doi":"10.1016/j.febslet.2007.09.037","title":"Role of Ser216 in the mechanism of action of membrane‐bound lytic transglycosylase B: Further evidence for substrate‐assisted catalysis","year":2007,"lang":"en","type":"article","venue":"FEBS Letters","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research","keywords":"Peptidoglycan; Lytic cycle; Glycosidic bond; Residue (chemistry); Chemistry; Enzyme; Stereochemistry; Biochemistry; Alanine; Bacterial cell structure; Mechanism of action; Substrate (aquarium); Amino acid; Bacteria; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002038363,0.0005471344,0.0003292936,0.0001279912,0.000261526,0.0004295713,0.0003663854,0.0007389709,0.001680987],"category_scores_gemma":[0.0002962143,0.0001148611,0.0003629932,0.0001374042,0.0002939838,0.0003576364,0.0002076323,0.0004498048,0.0007767369],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002754931,"about_ca_system_score_gemma":0.0002169282,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004300396,"about_ca_topic_score_gemma":0.0001823727,"domain_scores_codex":[0.9998962,0.00001958713,0.00001397482,0.00001772116,0.00003120401,0.00002134582],"domain_scores_gemma":[0.9998007,0.0000321825,0.00005502185,0.00002017715,0.00003115617,0.00006077494],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004067487,0.00003187709,0.000576398,0.00005717213,0.000007685837,0.0004406802,0.00002113282,0.0001364792,0.9969296,0.0001875097,0.00002824315,0.00117633],"study_design_scores_gemma":[0.00004393224,0.0002911449,0.003947403,0.000008018988,0.00001867279,0.001393617,0.00003220444,0.00132382,0.9909468,0.00008488448,0.001899045,0.00001051826],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9945374,0.002070131,0.001685649,0.0001636386,0.00005823489,0.00001460883,0.0001157404,0.00003660981,0.001317961],"genre_scores_gemma":[0.9963089,0.0009077604,0.001416462,0.00004688248,0.00001635251,0.000006951771,0.0002318801,0.0000111272,0.001053639],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001680987,"threshold_uncertainty_score":0.005623519,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03447912164142067,"score_gpt":0.2947148599207434,"score_spread":0.2602357382793227,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}