{"id":"W2041755586","doi":"10.1006/prep.2001.1437","title":"Expression, Purification, and Characterization of Recombinant Purine Nucleoside Phosphorylase from Escherichia coli","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":false,"ca_institutions":"Concordia University","funders":"","keywords":"Purine nucleoside phosphorylase; Escherichia coli; Recombinant DNA; Phosphorolysis; Biochemistry; Inosine; Chemistry; Nucleoside; Enzyme; Molecular biology; Biology; Purine; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001750264,0.0001558647,0.0001504759,0.00004509266,0.0001174266,0.0000368365,0.0001392285,0.0001994643,0.00005686753],"category_scores_gemma":[0.00009996089,0.0001360523,0.00003417565,0.0001077269,0.0001152409,0.00001732071,0.0001161188,0.0001072803,0.000004099222],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006255445,"about_ca_system_score_gemma":0.00002959975,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003455373,"about_ca_topic_score_gemma":0.000001345985,"domain_scores_codex":[0.9987797,0.0001083881,0.0003013584,0.0004635157,0.0001777629,0.0001693153],"domain_scores_gemma":[0.9991391,0.000009043138,0.0001853321,0.0003770872,0.0001443842,0.000145003],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002639431,0.0001366413,0.0008343483,0.00003484797,0.000008202194,8.05053e-7,0.00006039793,1.633999e-7,0.990431,0.000069466,0.0001919437,0.007968289],"study_design_scores_gemma":[0.0005430355,0.00009162448,0.01359386,0.0000929071,0.000007225652,0.000002174918,0.00003098903,0.00005519092,0.957269,0.0002610569,0.02789533,0.000157651],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9938951,0.001382118,0.003442397,0.0005202573,0.00004507661,0.0005742526,0.00002063253,0.00001705355,0.0001031819],"genre_scores_gemma":[0.9938012,0.002220688,0.002219168,0.00005932708,0.00007175122,0.000182159,0.0006265776,0.00002272653,0.0007964558],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03316199,"threshold_uncertainty_score":0.5548053,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01054247720899286,"score_gpt":0.2382473135139214,"score_spread":0.2277048363049285,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}