{"id":"W2041755586","doi":"10.1006/prep.2001.1437","title":"Expression, Purification, and Characterization of Recombinant Purine Nucleoside Phosphorylase from Escherichia coli","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":false,"ca_institutions":"Concordia University","funders":"","keywords":"Purine nucleoside phosphorylase; Escherichia coli; Recombinant DNA; Phosphorolysis; Biochemistry; Inosine; Chemistry; Nucleoside; Enzyme; Molecular biology; Biology; Purine; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004074851,0.0004587454,0.0005859992,0.0004499333,0.0003883909,0.000726991,0.0005357732,0.0003933671,0.0004882998],"category_scores_gemma":[0.0006788332,0.0003538162,0.0003411414,0.0009371883,0.0002512862,0.0003575906,0.0002506148,0.0009678035,0.0006941279],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006351341,"about_ca_system_score_gemma":0.0005344396,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003360911,"about_ca_topic_score_gemma":0.002536473,"domain_scores_codex":[0.999675,0.00005881999,0.00004407532,0.0000442892,0.0001091934,0.00006866478],"domain_scores_gemma":[0.9997047,0.0000767145,0.00003432619,0.00005922607,0.00007169109,0.00005335216],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000133861,0.00007734054,0.0003135053,0.0001004414,0.00001027663,0.0001436574,0.00005084767,0.0002547262,0.9958389,0.0002709496,0.000119009,0.002686359],"study_design_scores_gemma":[0.00001484789,0.00006236933,0.00352846,0.000009497095,0.00001806184,0.0003035605,0.00003580695,0.001121976,0.9896995,0.0001211361,0.00507388,0.00001087557],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.950449,0.004292249,0.03609803,0.0007690088,0.0001226595,0.0002065666,0.005050339,0.0003129441,0.00269912],"genre_scores_gemma":[0.9163288,0.005625854,0.04935554,0.00023256,0.0000533711,0.0001589063,0.01893421,0.0002121103,0.009098648],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003360911,"threshold_uncertainty_score":0.006682694,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01054247720899286,"score_gpt":0.2382473135139214,"score_spread":0.2277048363049285,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}