{"id":"W2042040522","doi":"10.1271/bbb.90013","title":"Directed Evolution of the Actinomycete Cytochrome P450 MoxA (CYP105) for Enhanced Activity","year":2009,"lang":"en","type":"article","venue":"Bioscience Biotechnology and Biochemistry","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"Nutrasource","funders":"","keywords":"Directed evolution; Mutagenesis; Mutant; Hydroxylation; Cytochrome P450; Naringenin; Enzyme; Active site; Biochemistry; Substrate (aquarium); Chemistry; Wild type; Enzyme assay; Site-directed mutagenesis; Stereochemistry; Mutation; Biology; Gene; Flavonoid; Antioxidant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000144616,0.0001262169,0.0001974056,0.00004942389,0.0001611479,0.00000710799,0.0001951835,0.0004196449,0.000005459445],"category_scores_gemma":[0.0005291132,0.0000774039,0.00007407951,0.0004326438,0.0006336818,0.00004131777,0.00005776384,0.0001800087,4.855376e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006438546,"about_ca_system_score_gemma":0.0001052066,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000896259,"about_ca_topic_score_gemma":9.628004e-7,"domain_scores_codex":[0.9991415,0.000008939804,0.0001479019,0.0003804743,0.00009715591,0.000224101],"domain_scores_gemma":[0.9993787,0.00002387819,0.0001407493,0.0003378478,0.00007643812,0.00004234586],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001213109,0.00007376156,0.00005139167,0.00004899054,0.00000704324,2.9957e-7,0.000005209482,2.068291e-8,0.9871957,0.00008308525,0.00008574846,0.01232741],"study_design_scores_gemma":[0.0002768968,0.0001844506,0.0212393,0.00006517728,0.00003108243,0.00003708766,0.00002074687,0.00002687399,0.9775399,0.0001040155,0.0003771992,0.0000972749],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9877293,0.000298705,0.000151485,0.01123361,0.00006036176,0.0003211227,0.00002415775,0.00009065426,0.00009062231],"genre_scores_gemma":[0.9985402,0.00006305076,0.000931378,0.000108445,0.0000549862,0.000001473068,0.000001684803,0.000003185567,0.0002955937],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02118791,"threshold_uncertainty_score":0.3236686,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007067490837530524,"score_gpt":0.2349019243287382,"score_spread":0.2278344334912076,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}