{"id":"W2042040522","doi":"10.1271/bbb.90013","title":"Directed Evolution of the Actinomycete Cytochrome P450 MoxA (CYP105) for Enhanced Activity","year":2009,"lang":"en","type":"article","venue":"Bioscience Biotechnology and Biochemistry","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"Nutrasource","funders":"","keywords":"Directed evolution; Mutagenesis; Mutant; Hydroxylation; Cytochrome P450; Naringenin; Enzyme; Active site; Biochemistry; Substrate (aquarium); Chemistry; Wild type; Enzyme assay; Site-directed mutagenesis; Stereochemistry; Mutation; Biology; Gene; Flavonoid; Antioxidant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000245253,0.00039097,0.0002826809,0.0002751338,0.0001174587,0.0002974774,0.0002108109,0.0002824848,0.0004789081],"category_scores_gemma":[0.0003240096,0.0001192387,0.0002440019,0.0002912995,0.0001223134,0.00008922256,0.0002300621,0.0003815508,0.0001736503],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002118365,"about_ca_system_score_gemma":0.00016748,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006084101,"about_ca_topic_score_gemma":0.001140832,"domain_scores_codex":[0.9997959,0.00005779264,0.00002384567,0.00002970692,0.00006442238,0.00002835462],"domain_scores_gemma":[0.9999045,0.00002548974,0.00002919104,0.00001213762,0.00001191276,0.00001671046],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009794971,0.00005907971,0.0007566824,0.00003196042,0.00001555873,0.0001351078,0.0000141607,0.0003461781,0.9928038,0.00009791375,0.00004429561,0.005597469],"study_design_scores_gemma":[0.00009175346,0.0008394464,0.01130013,0.0000190797,0.00006421663,0.00117421,0.00004350775,0.006335643,0.9755545,0.00007124903,0.004490399,0.00001587507],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9933328,0.0003651634,0.005028725,0.0000698978,0.00001624188,0.0000770086,0.0001850895,0.00007858455,0.0008465152],"genre_scores_gemma":[0.9907941,0.0002719272,0.007617291,0.00003807645,0.000006519258,0.00002852061,0.0004418894,0.00002017879,0.0007815422],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006084101,"threshold_uncertainty_score":0.001602054,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007067490837530524,"score_gpt":0.2349019243287382,"score_spread":0.2278344334912076,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}