{"id":"W2042333130","doi":"10.3791/52139","title":"Detection of &lt;em&gt;In Situ&lt;/em&gt; Protein-protein Complexes at the &lt;em&gt;Drosophila&lt;/em&gt; Larval Neuromuscular Junction Using Proximity Ligation Assay","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Cellular Mechanics and Interactions","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Yale University","keywords":"Proximity ligation assay; Neuromuscular junction; Biology; Immunoprecipitation; Postsynaptic potential; Cell biology; Drosophila melanogaster; Scaffold protein; Guanylate kinase; Molecular biology; Primary and secondary antibodies; Antibody; Membrane protein; Signal transduction; Biochemistry; Receptor; Neuroscience; Genetics; Gene; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006804714,0.001068963,0.0005275536,0.0008987449,0.0004880113,0.0005375241,0.0006824957,0.0007756478,0.002900058],"category_scores_gemma":[0.0004680204,0.0005691797,0.0005222771,0.0004873161,0.0005118706,0.0007591437,0.0007274855,0.00161605,0.001836303],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004434462,"about_ca_system_score_gemma":0.0002540186,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003319815,"about_ca_topic_score_gemma":0.0008058182,"domain_scores_codex":[0.999356,0.0001333033,0.0000562498,0.0002280828,0.0001385376,0.00008780947],"domain_scores_gemma":[0.9995431,0.0001366029,0.0001204952,0.00005503684,0.00006598912,0.00007881565],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002196129,0.00001242986,0.00007033494,0.00005453801,0.000006658844,0.00004903108,0.00002179677,0.00002233244,0.9987883,0.0001434183,0.00005265879,0.000756658],"study_design_scores_gemma":[0.000007508682,0.00008217599,0.001726753,0.000008901945,0.00002341413,0.0002454412,0.00003647338,0.001834499,0.9924558,0.0001046602,0.003467848,0.000006574891],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.531091,0.004639117,0.4509687,0.0005796792,0.0002056301,0.0002197767,0.0007474787,0.001823297,0.009725276],"genre_scores_gemma":[0.613754,0.005538536,0.3558359,0.0004848846,0.0001299805,0.0009000393,0.002715412,0.0005577014,0.02008356],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002900058,"threshold_uncertainty_score":0.009701669,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03296617585614717,"score_gpt":0.3312300111313985,"score_spread":0.2982638352752514,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}