{"id":"W2042333130","doi":"10.3791/52139","title":"Detection of <em>In Situ</em> Protein-protein Complexes at the <em>Drosophila</em> Larval Neuromuscular Junction Using Proximity Ligation Assay","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Cellular Mechanics and Interactions","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Yale University","keywords":"Proximity ligation assay; Neuromuscular junction; Biology; Immunoprecipitation; Postsynaptic potential; Cell biology; Drosophila melanogaster; Scaffold protein; Guanylate kinase; Molecular biology; Primary and secondary antibodies; Antibody; Membrane protein; Signal transduction; Biochemistry; Receptor; Neuroscience; Genetics; Gene; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001733999,0.0005054328,0.000658564,0.0003053407,0.0003471722,0.0001768499,0.0004270957,0.0003994225,0.0000682199],"category_scores_gemma":[0.000451615,0.0004321965,0.0005352108,0.0003894901,0.00008861936,0.0001377524,0.0003019938,0.0004442597,0.00001692455],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004123873,"about_ca_system_score_gemma":0.0002524552,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004124243,"about_ca_topic_score_gemma":0.0002229233,"domain_scores_codex":[0.9955241,0.0008279901,0.001373262,0.0005930488,0.00116496,0.0005166327],"domain_scores_gemma":[0.9966792,0.00002631801,0.001519923,0.0006310244,0.0008680867,0.0002754618],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001169921,0.0006859757,0.00002789533,0.00004492751,0.0002263776,0.00002186792,0.0005615128,0.0002376551,0.9958909,0.0001044723,0.0002162141,0.0008123199],"study_design_scores_gemma":[0.003281307,0.001461256,0.0003072723,0.0002147024,0.0001224753,0.0001766943,0.0004543771,0.002909487,0.980778,0.0002734642,0.009605592,0.0004153977],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9867166,0.001852565,0.008898669,0.00009794314,0.001101516,0.001167657,0.00001746331,0.00002164612,0.0001259485],"genre_scores_gemma":[0.9973643,0.00009587553,0.001189772,0.00009973913,0.0006157,0.00007680953,0.00009183555,0.0000903561,0.000375625],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01511289,"threshold_uncertainty_score":0.999813,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03296617585614717,"score_gpt":0.3312300111313985,"score_spread":0.2982638352752514,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}