{"id":"W2042754039","doi":"10.1016/j.pep.2014.05.013","title":"Over-expression in E. coli and purification of functional full-length murine small C-terminal domain phosphatase (SCP1, or Golli-interacting protein)","year":2014,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; Multiple Sclerosis Society of Canada","keywords":"Phosphatase; Gene isoform; Protein subunit; Recombinant DNA; Biology; Molecular biology; Biochemistry; Gene; Phosphorylation; Chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000371921,0.0008561026,0.0004901079,0.0004434623,0.0002295446,0.0005386429,0.0004729176,0.0003844388,0.001026603],"category_scores_gemma":[0.000232682,0.0002624877,0.0004354456,0.000998608,0.0003449599,0.0003086784,0.0003757356,0.0009688552,0.001176259],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007162304,"about_ca_system_score_gemma":0.0006643495,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001931425,"about_ca_topic_score_gemma":0.00201331,"domain_scores_codex":[0.9997897,0.00003047505,0.00002292222,0.00003707362,0.00006860281,0.00005124661],"domain_scores_gemma":[0.9998477,0.00003273245,0.00002735773,0.00002764858,0.00002621295,0.00003827078],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001090176,0.00005297086,0.0001897453,0.0001369756,0.00001529864,0.00007145719,0.00003114745,0.0001483767,0.9968459,0.0004032759,0.0002517837,0.001744008],"study_design_scores_gemma":[0.00003582432,0.0001228792,0.002814396,0.00001732605,0.00003927574,0.0003035542,0.00003547793,0.001633998,0.9851854,0.0001613099,0.009637043,0.00001344587],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9355786,0.005165142,0.04005878,0.0009147761,0.0002967636,0.0005644697,0.01151983,0.0009235695,0.004978168],"genre_scores_gemma":[0.8848792,0.004654111,0.05354423,0.0003180876,0.00005709123,0.0005131573,0.03594944,0.00019946,0.01988519],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001931425,"threshold_uncertainty_score":0.005196571,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01864222582742811,"score_gpt":0.2717074939661367,"score_spread":0.2530652681387087,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}