{"id":"W2043011805","doi":"10.3892/ol.2014.2684","title":"Optimization of lentiviral vector production using polyethylenimine-mediated transfection","year":2014,"lang":"en","type":"article","venue":"Oncology Letters","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":64,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"","keywords":"Polyethylenimine; Transfection; Molecular biology; Chemistry; Cell culture; Titer; Viral vector; Biology; Recombinant DNA; Gene; Virology; Biochemistry; Virus; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00185697,0.0007897756,0.0008651793,0.0008176149,0.0003371265,0.001134232,0.0007116022,0.0005579729,0.0007394578],"category_scores_gemma":[0.001183843,0.0003658623,0.0005411921,0.0006421773,0.0003881984,0.0005422374,0.0004578738,0.0008506411,0.0006262945],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005958271,"about_ca_system_score_gemma":0.000503343,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007113266,"about_ca_topic_score_gemma":0.001142599,"domain_scores_codex":[0.9985424,0.0002746215,0.0002628431,0.0002657198,0.0005262517,0.0001281833],"domain_scores_gemma":[0.9996805,0.000105258,0.00006586545,0.00003653233,0.00008959325,0.00002221057],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006813755,0.00006476191,0.0003284399,0.000207082,0.00002222752,0.000113292,0.00006925301,0.000655573,0.9893225,0.0003405435,0.0001471684,0.008660991],"study_design_scores_gemma":[0.00001557029,0.0002219875,0.001298794,0.00002905207,0.000040631,0.0001676586,0.00002067359,0.002556492,0.9881893,0.00008471846,0.007352883,0.0000222562],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7060003,0.009220228,0.2704921,0.0002305526,0.0003451558,0.002911675,0.001667188,0.00129528,0.007837436],"genre_scores_gemma":[0.6387526,0.009541545,0.3354279,0.0002193237,0.00007783762,0.002099726,0.005470454,0.0005198787,0.007890712],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00185697,"threshold_uncertainty_score":0.0098207,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01750220050616422,"score_gpt":0.2944259827862505,"score_spread":0.2769237822800862,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}