{"id":"W2043011805","doi":"10.3892/ol.2014.2684","title":"Optimization of lentiviral vector production using polyethylenimine-mediated transfection","year":2014,"lang":"en","type":"article","venue":"Oncology Letters","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":64,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"","keywords":"Polyethylenimine; Transfection; Molecular biology; Chemistry; Cell culture; Titer; Viral vector; Biology; Recombinant DNA; Gene; Virology; Biochemistry; Virus; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003518913,0.000102242,0.0001379103,0.00009204224,0.00005838368,0.00000481535,0.0000918251,0.0001429604,0.00002338574],"category_scores_gemma":[0.00008930008,0.0001067003,0.00006260156,0.0001311093,0.0001348308,0.000006271627,0.00001781631,0.00009355089,0.000003803041],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005145579,"about_ca_system_score_gemma":0.0000639479,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002226973,"about_ca_topic_score_gemma":0.00005893452,"domain_scores_codex":[0.9989628,0.0002884586,0.0001749713,0.0002654336,0.0001137642,0.0001945969],"domain_scores_gemma":[0.999602,0.00001666457,0.00008057365,0.0001970567,0.0000668909,0.00003680906],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009043198,0.00007070158,0.0004522707,0.00001284579,0.00004179718,5.342027e-7,0.00003933139,0.03010079,0.9654081,0.000001837605,0.0003353403,0.002632111],"study_design_scores_gemma":[0.0007765157,0.002276866,0.0003896191,0.000006774519,0.00002421006,0.0000106487,0.00001369361,0.004804926,0.989814,0.000002198535,0.001757286,0.0001232543],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9690329,0.0000533711,0.02861946,0.001626691,0.000337249,0.0001962748,0.000003853687,0.00001411604,0.0001160983],"genre_scores_gemma":[0.997436,0.00004095548,0.001511811,0.0004624506,0.000372994,0.00001401184,0.00009865249,0.00002272592,0.00004035397],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02840316,"threshold_uncertainty_score":0.4351111,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01750220050616422,"score_gpt":0.2944259827862505,"score_spread":0.2769237822800862,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}