{"id":"W2043302496","doi":"10.1093/nar/gkp1223","title":"A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI","year":2010,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Government of Ontario","keywords":"Homing endonuclease; Biology; Endonuclease; Computational biology; Function (biology); Linker; Homology (biology); Homology modeling; Cleavage (geology); Protein function; Mutant; Genetics; Amino acid; DNA; Biochemistry; Enzyme; Gene; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002190309,0.001658099,0.001228879,0.001720922,0.0006865436,0.001660081,0.001710749,0.001045379,0.0006048863],"category_scores_gemma":[0.002772699,0.0006224726,0.0009168652,0.0009941075,0.001444385,0.002451763,0.001568863,0.001770413,0.0002344211],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001229095,"about_ca_system_score_gemma":0.002650506,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001084806,"about_ca_topic_score_gemma":0.001769831,"domain_scores_codex":[0.9993698,0.0001690414,0.00006169381,0.0001863674,0.0001703312,0.00004275292],"domain_scores_gemma":[0.9991487,0.0003599781,0.000187342,0.00017025,0.00008029181,0.00005337764],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003611822,0.0004253663,0.012547,0.002125653,0.0004269226,0.0005138137,0.0001913182,0.2588221,0.4765686,0.1375625,0.001030527,0.1094251],"study_design_scores_gemma":[0.00005954302,0.0004999173,0.004801919,0.0001579417,0.0002574869,0.0004115468,0.0002403725,0.6968652,0.1773751,0.1107783,0.008415595,0.000137115],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.0689075,0.001724917,0.9251682,0.000622189,0.00003257745,0.0001128668,0.0005003808,0.001189695,0.001741704],"genre_scores_gemma":[0.363306,0.0024822,0.6320279,0.0001549123,0.00004312192,0.000265875,0.001205971,0.0001606875,0.0003533286],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002190309,"threshold_uncertainty_score":0.01158357,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0159695969630964,"score_gpt":0.3398975350941449,"score_spread":0.3239279381310485,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}