{"id":"W2043302496","doi":"10.1093/nar/gkp1223","title":"A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI","year":2010,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Government of Ontario","keywords":"Homing endonuclease; Biology; Endonuclease; Computational biology; Function (biology); Linker; Homology (biology); Homology modeling; Cleavage (geology); Protein function; Mutant; Genetics; Amino acid; DNA; Biochemistry; Enzyme; Gene; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003311457,0.00009996205,0.00009162793,0.00006363853,0.0002071901,0.00004683801,0.0002460408,0.0001348531,0.00008136847],"category_scores_gemma":[0.0003184664,0.00008266377,0.00005552994,0.000120875,0.0003636776,0.000004242015,0.0003400617,0.0003416736,0.00000526751],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007949583,"about_ca_system_score_gemma":0.00007785951,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002374987,"about_ca_topic_score_gemma":0.00002641387,"domain_scores_codex":[0.9988589,0.00007364917,0.0001783134,0.0002691915,0.0003935743,0.000226388],"domain_scores_gemma":[0.9993029,0.00008520979,0.00003329508,0.000334855,0.0001643028,0.00007940494],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007598471,0.00004255731,0.005139694,0.00002121116,0.00002870522,0.000001910498,0.000226062,0.000457347,0.9918352,0.000709909,0.0006669126,0.000794523],"study_design_scores_gemma":[0.0004431027,0.0001758629,0.1972325,0.00003364744,0.000008288445,0.00003500483,0.0006257074,0.000778052,0.7948681,0.001786983,0.003857396,0.0001554036],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9966122,0.0007216102,0.001650275,0.0002063728,0.0001954864,0.0001668654,0.00001156897,0.000008182527,0.0004274275],"genre_scores_gemma":[0.9925232,0.00004535276,0.006850244,0.00003519605,0.0001963166,0.00001449884,0.000007944546,0.00002191797,0.0003053238],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1969671,"threshold_uncertainty_score":0.3370931,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0159695969630964,"score_gpt":0.3398975350941449,"score_spread":0.3239279381310485,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}