{"id":"W2043911972","doi":"10.1002/(sici)1097-0290(20000720)69:2<204::aid-bit9>3.0.co;2-z","title":"Limitations to the amplification and stability of human tissue-type plasminogen activator expression by Chinese hamster ovary cells","year":2000,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":97,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Chinese hamster ovary cell; Molecular biology; Plasminogen activator; Biology; T-plasminogen activator; Cell culture; Complementary DNA; Subcloning; Microgram; Tissue plasminogen activator; Recombinant DNA; In vitro; Endocrinology; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002176274,0.0003579661,0.0004799826,0.0003537772,0.000214378,0.001359702,0.0005065374,0.0005626995,0.001273976],"category_scores_gemma":[0.004891263,0.0002788391,0.0004866229,0.0003266799,0.0004405057,0.0003944258,0.0004760245,0.0006858847,0.001675824],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000567904,"about_ca_system_score_gemma":0.0003523494,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00125289,"about_ca_topic_score_gemma":0.000824798,"domain_scores_codex":[0.9975266,0.0008021169,0.0003706175,0.0002786026,0.0009029687,0.0001191599],"domain_scores_gemma":[0.9950505,0.003049542,0.0004068772,0.0007044146,0.00068349,0.0001051833],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005276373,0.00001152142,0.0009429481,0.0001441315,0.00001193198,0.00005100315,0.00009532816,0.0001377985,0.9918028,0.0002376956,0.0001311681,0.006381052],"study_design_scores_gemma":[0.000006540232,0.0002315557,0.005494277,0.00004732495,0.00006338905,0.001072992,0.00008891132,0.001702376,0.9726949,0.0002305265,0.01835434,0.00001295613],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8375179,0.02435261,0.1258781,0.001869254,0.000288276,0.000463928,0.001286864,0.0008292224,0.007513907],"genre_scores_gemma":[0.9398205,0.009071882,0.04089971,0.0004965139,0.0001292355,0.0004150444,0.002272827,0.000161052,0.006733172],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002176274,"threshold_uncertainty_score":0.01150936,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01395236639231321,"score_gpt":0.2440886946954828,"score_spread":0.2301363283031696,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}