{"id":"W2043995661","doi":"10.1016/j.pep.2005.09.021","title":"An efficient mammalian cell-free translation system supplemented with translation factors","year":2005,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Viral Infections and Immunology Research","field":"Medicine","cited_by":129,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University","funders":"RIKEN; Ministry of Education, Culture, Sports, Science and Technology","keywords":"EIF4G; EIF4E; Internal ribosome entry site; Eukaryotic translation; Reticulocyte; Initiation factor; Eukaryotic initiation factor; Protein biosynthesis; Eukaryotic translation initiation factor 4 gamma; Translation (biology); EIF4EBP1; EIF4A1; eIF2; HeLa; Cell biology; Biology; Messenger RNA; Molecular biology; Cell; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002313419,0.0001342496,0.0001413365,0.0001722055,0.0002468361,0.00004163563,0.0000703795,0.0001181821,0.0001101554],"category_scores_gemma":[0.000006414463,0.00009570549,0.00003048623,0.0001737739,0.00005510497,0.0001999283,0.000006304823,0.0001745539,0.0000106734],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004973423,"about_ca_system_score_gemma":0.00003327313,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003922562,"about_ca_topic_score_gemma":0.000009384331,"domain_scores_codex":[0.9989266,0.0001236505,0.0002398011,0.0002856734,0.0002408237,0.0001834307],"domain_scores_gemma":[0.999329,0.00001461714,0.00008057158,0.000367656,0.00009559836,0.0001125963],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004855307,0.0003499738,0.00213111,0.0001602087,0.00001019473,8.748729e-7,0.0009366514,0.0001299912,0.9668688,0.000188309,0.00003562505,0.02870273],"study_design_scores_gemma":[0.004081583,0.001013252,0.01600268,0.0002470124,0.0000537243,0.00001616078,0.001340737,0.02196367,0.9467148,0.000008300585,0.008341108,0.000217022],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.975185,0.0005382903,0.02031503,0.001216192,0.00002154228,0.001563746,0.00001912638,0.0001339982,0.001007096],"genre_scores_gemma":[0.991679,0.00002077071,0.007607078,0.00001123043,0.00005334362,0.0001768739,0.0002613208,0.00001850677,0.0001719025],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02848571,"threshold_uncertainty_score":0.3902757,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02725441943076696,"score_gpt":0.2873584739905042,"score_spread":0.2601040545597373,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}