{"id":"W2044150090","doi":"10.1016/j.pep.2008.10.011","title":"Transient expression and purification of chimeric heavy chain antibodies","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":105,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute; Institute for Biological Sciences","funders":"","keywords":"Single-domain antibody; Monoclonal antibody; Antibody; Fusion protein; Glycosylation; Molecular biology; Protein engineering; Recombinant DNA; In vitro; Heavy chain; Chemistry; Biology; Computational biology; Biochemistry; Immunology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008921704,0.0007415497,0.001041188,0.0007022176,0.0008515688,0.001144228,0.001405479,0.0006651988,0.002850868],"category_scores_gemma":[0.0005415541,0.0006792739,0.0009156718,0.001320499,0.0006121207,0.0005714627,0.0008270717,0.00330578,0.002214188],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001277237,"about_ca_system_score_gemma":0.0008391825,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001935302,"about_ca_topic_score_gemma":0.00277793,"domain_scores_codex":[0.9992431,0.0000786061,0.00008005608,0.0001734761,0.0002171583,0.0002075521],"domain_scores_gemma":[0.9993975,0.0001298844,0.00006500734,0.0001470482,0.0001450488,0.0001155537],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001220438,0.00007880227,0.0001268777,0.00008143432,0.00001401396,0.00005145961,0.00006662354,0.000101342,0.9961552,0.0009351466,0.0004962459,0.001770833],"study_design_scores_gemma":[0.00005170418,0.00009716045,0.001433506,0.00001563701,0.00003468326,0.0001989998,0.00005262339,0.001886346,0.9788181,0.0002628017,0.01713551,0.00001297422],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6609389,0.003915304,0.3000081,0.001534888,0.0008308316,0.001403174,0.01526468,0.002661834,0.01344224],"genre_scores_gemma":[0.7323973,0.003903333,0.177319,0.0005769235,0.0001777568,0.001864372,0.04252122,0.001276108,0.03996402],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002850868,"threshold_uncertainty_score":0.009537101,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03438710050932131,"score_gpt":0.2952148807357156,"score_spread":0.2608277802263943,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}