{"id":"W2044150090","doi":"10.1016/j.pep.2008.10.011","title":"Transient expression and purification of chimeric heavy chain antibodies","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":105,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute; Institute for Biological Sciences","funders":"","keywords":"Single-domain antibody; Monoclonal antibody; Antibody; Fusion protein; Glycosylation; Molecular biology; Protein engineering; Recombinant DNA; In vitro; Heavy chain; Chemistry; Biology; Computational biology; Biochemistry; Immunology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002538022,0.0001787173,0.0003023878,0.0001742674,0.0002787393,0.00001360901,0.00007707792,0.0001225605,0.00007496749],"category_scores_gemma":[0.00007015398,0.0001323362,0.00005820516,0.0001682178,0.0002977652,0.0001418828,0.00004208357,0.0001947195,0.000007985007],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001240235,"about_ca_system_score_gemma":0.00004950766,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005050873,"about_ca_topic_score_gemma":6.678978e-7,"domain_scores_codex":[0.998469,0.000118492,0.0003848326,0.0003848573,0.0004248119,0.0002180104],"domain_scores_gemma":[0.9991797,0.00004813799,0.0001591027,0.0002775185,0.0001397625,0.0001958081],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006604686,0.0002133257,0.005146946,0.0003719472,0.00001050136,0.00000493124,0.001302241,8.31173e-7,0.9791864,0.0003042408,0.0003645767,0.01243359],"study_design_scores_gemma":[0.0008529452,0.0002621599,0.1168058,0.0004851508,0.00001457229,0.00007915041,0.0001625433,0.0003176631,0.8725343,0.0001519438,0.008176275,0.0001575515],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.99005,0.005113499,0.0009245135,0.001899064,0.00003326045,0.001006103,0.00001585808,0.0000441799,0.0009134942],"genre_scores_gemma":[0.9883254,0.004072972,0.005479174,0.00005479994,0.00008297053,0.0001229806,0.00007396924,0.0000176278,0.001770147],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1116588,"threshold_uncertainty_score":0.5396515,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03438710050932131,"score_gpt":0.2952148807357156,"score_spread":0.2608277802263943,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}