{"id":"W2044200819","doi":"10.1021/ac0709495","title":"Two-Photon Excitation Fluorescence Cross-Correlation Assay for Ligand−Receptor Binding:  Cell Membrane Nanopatches Containing the Human μ-Opioid Receptor","year":2007,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Regina; University of Calgary","funders":"","keywords":"Chemistry; Receptor; Ligand (biochemistry); Dissociation constant; Opioid receptor; Biophysics; G protein-coupled receptor; Fluorescence; Stereochemistry; Enkephalin; Fluorescence correlation spectroscopy; Cell membrane; Membrane; Biochemistry; Opioid; Molecule","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001235008,0.0008043767,0.0008300138,0.0007817068,0.0007019801,0.0005636011,0.001031733,0.001255716,0.001655816],"category_scores_gemma":[0.001727307,0.0003707913,0.0003377314,0.0008036673,0.0005084046,0.0004560226,0.0004294714,0.001452579,0.0007305235],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001081406,"about_ca_system_score_gemma":0.0006943813,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002632783,"about_ca_topic_score_gemma":0.00385479,"domain_scores_codex":[0.9987926,0.0003458705,0.00006737909,0.000291494,0.0003812439,0.0001212973],"domain_scores_gemma":[0.9978363,0.001358042,0.0001949665,0.0002399309,0.0002657959,0.0001050697],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001221986,0.0001647955,0.0005648077,0.00008649109,0.00001905028,0.00005118161,0.00004218967,0.0008865235,0.9935187,0.0004738449,0.0001955444,0.003874654],"study_design_scores_gemma":[0.000008972881,0.0001178808,0.001309969,0.000003386554,0.000007521995,0.0001005124,0.0000136593,0.01379037,0.9842411,0.00006172017,0.0003341253,0.00001087166],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6650451,0.0007054952,0.327878,0.0001529703,0.0000500814,0.0003908593,0.0008571211,0.001146638,0.003773704],"genre_scores_gemma":[0.7405878,0.001025842,0.2516249,0.0002241516,0.00002379845,0.0009792357,0.001103449,0.00007350458,0.0043572],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002632783,"threshold_uncertainty_score":0.007846177,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01426578333319863,"score_gpt":0.2864602967625357,"score_spread":0.2721945134293371,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}