{"id":"W2044200819","doi":"10.1021/ac0709495","title":"Two-Photon Excitation Fluorescence Cross-Correlation Assay for Ligand−Receptor Binding:  Cell Membrane Nanopatches Containing the Human μ-Opioid Receptor","year":2007,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Regina; University of Calgary","funders":"","keywords":"Chemistry; Receptor; Ligand (biochemistry); Dissociation constant; Opioid receptor; Biophysics; G protein-coupled receptor; Fluorescence; Stereochemistry; Enkephalin; Fluorescence correlation spectroscopy; Cell membrane; Membrane; Biochemistry; Opioid; Molecule","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001306049,0.0002731532,0.0002340815,0.00002951775,0.0004020389,0.0001259034,0.0003131228,0.0003348013,0.000219596],"category_scores_gemma":[0.0003414746,0.0002286652,0.0002278564,0.0001494064,0.0001504585,0.00001553315,0.00006899734,0.0002350893,0.00002545469],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006154829,"about_ca_system_score_gemma":0.00007426419,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001809518,"about_ca_topic_score_gemma":0.000004282089,"domain_scores_codex":[0.9981459,0.00003246086,0.0004925859,0.0005540318,0.0002823044,0.0004927423],"domain_scores_gemma":[0.9988714,0.0001744741,0.000215118,0.0003413949,0.0002325112,0.0001650555],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001545619,0.00005646818,0.001555481,0.00007102996,0.00003944617,9.503871e-7,0.00009932361,0.00004600217,0.9970586,0.0002213637,0.0003913766,0.0003054239],"study_design_scores_gemma":[0.0008501704,0.0001376877,0.00008474151,0.00003619592,0.00004580904,0.000003120999,0.000463761,0.001162093,0.9926901,0.0001089147,0.004109223,0.0003081688],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9826966,0.000122089,0.01463233,0.00006041468,0.0002174291,0.0002870691,0.0000329252,0.00003126372,0.001919877],"genre_scores_gemma":[0.9928208,0.0000241428,0.001643312,0.00009908011,0.0007510317,0.00003603829,0.0008381441,0.00004473502,0.003742729],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01298902,"threshold_uncertainty_score":0.9324695,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01426578333319863,"score_gpt":0.2864602967625357,"score_spread":0.2721945134293371,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}