{"id":"W2044626588","doi":"10.1139/w99-127","title":"PCR cloning, heterologous expression, and characterization of isopenicillin N synthase from<i>Streptomyces lipmanii</i>NRRL 3584","year":2000,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National University of Singapore","keywords":"Biology; Cloning (programming); Streptomyces; Heterologous expression; Gene; Primer (cosmetics); Escherichia coli; ATP synthase; Biochemistry; Molecular biology; Homology (biology); Nucleic acid sequence; Genetics; Bacteria; Chemistry; Recombinant DNA","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001684087,0.0003618663,0.0003592938,0.0002562877,0.0001230453,0.0003186129,0.0002185484,0.0001888086,0.0006319191],"category_scores_gemma":[0.0003012857,0.0001433915,0.0002678209,0.0003750599,0.0002031622,0.0001606005,0.0002044607,0.0003986953,0.0006734982],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004115414,"about_ca_system_score_gemma":0.0003667275,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001497584,"about_ca_topic_score_gemma":0.001216731,"domain_scores_codex":[0.999774,0.00003945189,0.00002921391,0.00004976994,0.00007444803,0.00003310158],"domain_scores_gemma":[0.9998517,0.00003441664,0.00004290435,0.00002383632,0.00002312169,0.00002399907],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004485374,0.00002460061,0.0002641637,0.00004291757,0.00000264669,0.00008430208,0.00002364758,0.000129541,0.9971607,0.000122839,0.00002763591,0.00207224],"study_design_scores_gemma":[0.00002053437,0.0002131061,0.008023131,0.00001046052,0.00002329988,0.0006154447,0.00008432371,0.001385935,0.9831085,0.00008074737,0.006424041,0.00001057475],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9786038,0.0009079847,0.015915,0.000173773,0.00004923864,0.0001477949,0.001693516,0.0001676577,0.002341236],"genre_scores_gemma":[0.9590917,0.0009585497,0.02357606,0.00006474324,0.00002086531,0.0001009896,0.01078508,0.00006925596,0.005332856],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001497584,"threshold_uncertainty_score":0.002985954,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00846942993764352,"score_gpt":0.2042654442450161,"score_spread":0.1957960143073726,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}