{"id":"W2045131958","doi":"10.1096/fj.14-264499","title":"Solution NMR and calorimetric analysis of Rem2 binding to the Ca <sup>2+</sup> channel β4 subunit: a low affinity interaction is required for inhibition of Cav2.1 Ca <sup>2+</sup> currents","year":2015,"lang":"en","type":"article","venue":"The FASEB Journal","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"National Institute of Neurological Disorders and Stroke; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Alberta Innovates - Health Solutions; National Institutes of Health; National Science Foundation","keywords":"Protein subunit; Mutant; Binding site; Chemistry; Biophysics; Intracellular; Allosteric regulation; Wild type; Biochemistry; Biology; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001219135,0.0001651073,0.000252108,0.0005163238,0.0002784342,0.00004613981,0.0001379615,0.0001395276,0.00002366626],"category_scores_gemma":[0.0004316573,0.0001152656,0.0002196735,0.0008666012,0.00007194898,0.0000358254,0.00007945004,0.0001747125,0.000004763277],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00009386146,"about_ca_system_score_gemma":0.000091561,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00014724,"about_ca_topic_score_gemma":0.00002792229,"domain_scores_codex":[0.9985576,0.0002141853,0.000461527,0.000221791,0.0003150322,0.0002298998],"domain_scores_gemma":[0.9985633,0.00005388392,0.0003912096,0.0002471156,0.0005995041,0.0001449302],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"simulation_or_modeling","study_design_gemma":"simulation_or_modeling","study_design_scores_codex":[0.004188673,0.0005500057,0.001619331,0.0001459009,0.002190144,0.000003221782,0.01934644,0.7034258,0.221503,0.00004648898,0.03677671,0.01020421],"study_design_scores_gemma":[0.002359596,0.001116887,0.0005592777,0.0001215171,0.001002656,0.0000808456,0.008698757,0.7556247,0.2276429,0.0001504845,0.002322426,0.0003199112],"study_design_candidate":"simulation_or_modeling","study_design_consensus":"simulation_or_modeling","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9758363,0.0001792542,0.02229991,0.0008593026,0.0003120032,0.0002924825,0.0001727527,0.000005351804,0.00004264788],"genre_scores_gemma":[0.9986165,0.0001069884,0.00008739577,0.0001435689,0.0005520922,0.00001342032,0.0003006966,0.00001488311,0.000164459],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05219887,"threshold_uncertainty_score":0.4700395,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04674934310449742,"score_gpt":0.2901628367925167,"score_spread":0.2434134936880193,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}