{"id":"W2045131958","doi":"10.1096/fj.14-264499","title":"Solution NMR and calorimetric analysis of Rem2 binding to the Ca <sup>2+</sup> channel β4 subunit: a low affinity interaction is required for inhibition of Cav2.1 Ca <sup>2+</sup> currents","year":2015,"lang":"en","type":"article","venue":"The FASEB Journal","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"National Institute of Neurological Disorders and Stroke; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Alberta Innovates - Health Solutions; National Institutes of Health; National Science Foundation","keywords":"Protein subunit; Mutant; Binding site; Chemistry; Biophysics; Intracellular; Allosteric regulation; Wild type; Biochemistry; Biology; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001303744,0.0002740123,0.0002185286,0.00009997034,0.0001473137,0.0001401819,0.0002085046,0.0002035118,0.001195878],"category_scores_gemma":[0.0001905761,0.0001215137,0.0001731892,0.00009304898,0.0001900959,0.0001048905,0.0001223143,0.0003890904,0.0002003181],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000293048,"about_ca_system_score_gemma":0.000114605,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00125644,"about_ca_topic_score_gemma":0.001401445,"domain_scores_codex":[0.9999217,0.00001527055,0.000004930641,0.00001725278,0.00002166416,0.00001919574],"domain_scores_gemma":[0.9999079,0.00002244254,0.00002267588,0.000007290877,0.00001715231,0.0000226192],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008776338,0.00001239132,0.0002232379,0.00001529317,0.000004638899,0.00001936869,0.00001023459,0.0001262836,0.9992687,0.00002521554,0.00002337757,0.0001834467],"study_design_scores_gemma":[0.00001350242,0.00009369641,0.004359776,0.000003082062,0.00001031938,0.00008994967,0.00003812477,0.00425514,0.9906721,0.00002117376,0.0004361533,0.000007118037],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9975681,0.0001828354,0.001593566,0.00004248863,0.000008998658,0.000006023251,0.0001858661,0.00002106791,0.0003910009],"genre_scores_gemma":[0.9967324,0.0001287438,0.001984782,0.00003851293,0.000004413973,0.00001353559,0.0004065231,0.00001493829,0.0006763615],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00125644,"threshold_uncertainty_score":0.004000604,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04674934310449742,"score_gpt":0.2901628367925167,"score_spread":0.2434134936880193,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}