{"id":"W2046843537","doi":"10.1016/j.bbrc.2007.01.190","title":"Single cell transfection using plasmid decorated AFM probes","year":2007,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Force Microscopy Techniques and Applications","field":"Physics and Astronomy","cited_by":63,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Sherbrooke","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Transfection; Fluorescence microscope; Biophysics; Cell; Green fluorescent protein; Cell biology; Membrane; Cell membrane; Plasmid; Atomic force microscopy; Chemistry; DNA; Nanotechnology; Fluorescence; Materials science; Cell culture; Biology; Gene; Optics; Biochemistry; Genetics; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006971117,0.000605804,0.001007302,0.0005276811,0.0005681379,0.001356787,0.0007698261,0.001294145,0.00337058],"category_scores_gemma":[0.0004702868,0.0005085834,0.0005892866,0.0008044288,0.0006260521,0.0006465058,0.0007702884,0.001993337,0.002118561],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005989399,"about_ca_system_score_gemma":0.0003109361,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001027943,"about_ca_topic_score_gemma":0.001886867,"domain_scores_codex":[0.9991842,0.00009678736,0.0001050147,0.0002511405,0.0002053546,0.0001574447],"domain_scores_gemma":[0.9994393,0.0002306234,0.00007759197,0.0001380384,0.00006307039,0.00005145584],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006788872,0.00004405676,0.0001003304,0.00006503776,0.0000116843,0.00005440621,0.00007393833,0.00009334486,0.9968289,0.0007019913,0.0002150146,0.001743502],"study_design_scores_gemma":[0.00002090896,0.00007433691,0.0005554412,0.000003695924,0.00001808483,0.0000719477,0.00001909143,0.001822245,0.9921061,0.00007094386,0.005228883,0.000008284798],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7867655,0.002050645,0.1854171,0.001036887,0.00105817,0.001006437,0.005971672,0.00387594,0.01281771],"genre_scores_gemma":[0.8848643,0.001108069,0.07834503,0.0002859862,0.00008394744,0.0007384843,0.005921556,0.0005544364,0.02809824],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00337058,"threshold_uncertainty_score":0.01127571,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08071014475301361,"score_gpt":0.3926020897556761,"score_spread":0.3118919450026625,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}