{"id":"W2046843537","doi":"10.1016/j.bbrc.2007.01.190","title":"Single cell transfection using plasmid decorated AFM probes","year":2007,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Force Microscopy Techniques and Applications","field":"Physics and Astronomy","cited_by":63,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Sherbrooke","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Transfection; Fluorescence microscope; Biophysics; Cell; Green fluorescent protein; Cell biology; Membrane; Cell membrane; Plasmid; Atomic force microscopy; Chemistry; DNA; Nanotechnology; Fluorescence; Materials science; Cell culture; Biology; Gene; Optics; Biochemistry; Genetics; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003478454,0.0001198208,0.0001249518,0.00008372872,0.0005280354,0.00008046114,0.0004113458,0.00007342381,0.0000394498],"category_scores_gemma":[0.00001105314,0.0001078847,0.00006689185,0.0006095948,0.0006065213,0.00007773929,0.0002059398,0.0004106312,0.00002020483],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004928527,"about_ca_system_score_gemma":0.00004380177,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003130045,"about_ca_topic_score_gemma":0.00000509441,"domain_scores_codex":[0.998913,0.00006156083,0.0002150696,0.0002505642,0.0001890567,0.0003707355],"domain_scores_gemma":[0.9987546,0.0002489147,0.0000361392,0.0005822809,0.0002062419,0.0001718323],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001360147,0.0006581705,0.0002223109,0.0000104793,0.000007594018,9.019459e-8,0.00004485396,2.608213e-7,0.9841509,0.01143961,0.0004665673,0.0029855],"study_design_scores_gemma":[0.0001571887,0.00007510477,0.0001516647,0.00002512177,0.00001125878,5.795686e-7,0.0001596751,0.001028842,0.9863245,0.00387441,0.008056759,0.0001348481],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9709511,0.0001319901,0.02066618,0.000528137,0.00001020241,0.0004023285,0.00004518393,0.00007137145,0.007193467],"genre_scores_gemma":[0.9868399,0.00003047453,0.01273572,0.00001649469,0.00009439076,0.0000515062,0.0000997119,0.00001565756,0.0001161595],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01588875,"threshold_uncertainty_score":0.4399412,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08071014475301361,"score_gpt":0.3926020897556761,"score_spread":0.3118919450026625,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}