{"id":"W2047288355","doi":"10.1074/jbc.m705672200","title":"Activation of the SspA Serine Protease Zymogen of Staphylococcus aureus Proceeds through Unique Variations of a Trypsinogen-like Mechanism and Is Dependent on Both Autocatalytic and Metalloprotease-specific Processing","year":2007,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan; University of Toronto; Sunnybrook Health Science Centre","funders":"Canadian Institutes of Health Research; University of Toronto","keywords":"Zymogen; Endopeptidase; Protein precursor; Active site; Chemistry; Biochemistry; Proteolysis; Cleavage (geology); Serine; Protease; Enzyme; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001205691,0.0002803863,0.0001524571,0.0001504936,0.000100574,0.0002556028,0.0001291322,0.0001541207,0.0008581635],"category_scores_gemma":[0.0001161871,0.00009913845,0.0004155098,0.0001140256,0.000122339,0.0002105084,0.000197943,0.000311758,0.0005461921],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001902288,"about_ca_system_score_gemma":0.0001769723,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002887039,"about_ca_topic_score_gemma":0.0002970449,"domain_scores_codex":[0.9998876,0.00001611317,0.00001337434,0.00002398938,0.00003724918,0.00002159342],"domain_scores_gemma":[0.999899,0.00001374399,0.00002907724,0.00001579463,0.00001975109,0.0000225105],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003080885,0.000008065386,0.0004382612,0.00001910859,0.000003539099,0.00007473044,0.000009504598,0.00003710032,0.9977702,0.0001113092,0.00001798444,0.001479416],"study_design_scores_gemma":[0.00001124739,0.0002610986,0.02264659,0.000007154268,0.00001125069,0.001524319,0.00004018096,0.001869595,0.9709224,0.0001635478,0.002533216,0.000009439177],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9855012,0.001063185,0.01101885,0.000048112,0.00001981337,0.00004757752,0.0002366738,0.0001017556,0.001962793],"genre_scores_gemma":[0.9907829,0.0004642465,0.006484789,0.00003066212,0.000007460701,0.00003215656,0.0004222826,0.0000135542,0.001762021],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008581635,"threshold_uncertainty_score":0.002870798,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01755927482999252,"score_gpt":0.239257810995396,"score_spread":0.2216985361654035,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}