{"id":"W2047836010","doi":"10.1016/j.pep.2014.07.007","title":"Improved soluble expression of a single-chain antibody fragment in E. coli for targeting CA125 in epithelial ovarian cancer","year":2014,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":20,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"National Institutes of Health; University of Alberta; Iowa State University","keywords":"Single-chain variable fragment; Molecular biology; Heterologous; Linker; Escherichia coli; Codon usage bias; Expression vector; Monoclonal antibody; Recombinant DNA; Peptide; Chemistry; Biology; Gene; Antibody; Computational biology; Biochemistry; Genetics; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007347164,0.0001399731,0.0002984064,0.0001889126,0.00007954977,0.00001503034,0.00008329588,0.0001340725,0.00005671816],"category_scores_gemma":[0.0002430746,0.00010898,0.00004720942,0.0001451838,0.00007048334,0.00008507083,0.00005359712,0.0001738362,9.891871e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000460564,"about_ca_system_score_gemma":0.00006435114,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007402097,"about_ca_topic_score_gemma":0.00003792236,"domain_scores_codex":[0.998567,0.0001351832,0.000447898,0.0003340729,0.0002351491,0.0002806456],"domain_scores_gemma":[0.9993451,0.0001009449,0.0001694189,0.0001703998,0.0001073274,0.0001067656],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001324941,0.000291414,0.004081614,0.0005214149,0.000003385494,8.880588e-7,0.0006581995,0.00001210008,0.9831716,0.0002401677,0.00007529383,0.009619008],"study_design_scores_gemma":[0.00258321,0.0002898955,0.007435018,0.001278224,0.000005424498,9.285479e-7,0.0001651645,0.009954973,0.9718634,0.0004269876,0.005865039,0.0001317407],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9904349,0.0007515698,0.004827992,0.001649647,0.00007717338,0.002010759,0.00002171694,0.00001603098,0.0002101585],"genre_scores_gemma":[0.9906211,0.0001422251,0.007620042,0.00005904161,0.0001358062,0.000625153,0.00008549873,0.00001774744,0.0006934089],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01130818,"threshold_uncertainty_score":0.4444077,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02633578617446132,"score_gpt":0.3155660295759106,"score_spread":0.2892302434014493,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}