{"id":"W2048098763","doi":"10.1163/156854107781352098","title":"Application of a real-time PCR method for the detection of pine wood nematode, Bursaphelenchus xylophilus, in wood samples from lodgepole pine","year":2007,"lang":"en","type":"article","venue":"Nematology","topic":"Nematode management and characterization studies","field":"Agricultural and Biological Sciences","cited_by":63,"is_retracted":false,"has_abstract":true,"ca_institutions":"Natural Resources Canada; Canadian Food Inspection Agency; Canadian Forest Service","funders":"","keywords":"Bursaphelenchus xylophilus; Xylophilus; Biology; Pinus contorta; TaqMan; Nematode; Polymerase chain reaction; Botany; Primer (cosmetics); DNA extraction; Real-time polymerase chain reaction; Population; Ecology; Genetics; Gene; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004947093,0.0004685719,0.0002816192,0.0005409206,0.0001419497,0.0003728083,0.0003879477,0.0003388772,0.0005959532],"category_scores_gemma":[0.0008851545,0.0002970072,0.0001702143,0.000248587,0.0002916185,0.000258594,0.0001181901,0.0003401568,0.0003202748],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001904371,"about_ca_system_score_gemma":0.000248855,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005351446,"about_ca_topic_score_gemma":0.001408648,"domain_scores_codex":[0.9994326,0.0001186948,0.00003560706,0.0002128646,0.000158325,0.00004182305],"domain_scores_gemma":[0.9995208,0.0001920648,0.0001239648,0.00002805344,0.00009561107,0.00003957554],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005268737,0.00002396936,0.001259198,0.00004910342,0.000004072559,0.00003848262,0.00004194519,0.0000675614,0.9940351,0.0000272397,0.00002189911,0.004378869],"study_design_scores_gemma":[0.0000383522,0.001356816,0.03372364,0.00004766053,0.00009928014,0.0009875676,0.0002545144,0.007815764,0.9522383,0.0001059803,0.003297918,0.00003421587],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8588664,0.002094745,0.1358713,0.0001206972,0.0001161636,0.0003075218,0.0008506653,0.0004366352,0.001335822],"genre_scores_gemma":[0.7235596,0.001487853,0.270089,0.0001393974,0.00003368132,0.0006220121,0.001323703,0.00002949623,0.002715343],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005959532,"threshold_uncertainty_score":0.002616286,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01900754359012196,"score_gpt":0.264419240464446,"score_spread":0.245411696874324,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}