{"id":"W2048100862","doi":"10.3791/2094","title":"An Assay for Measuring the Activity of <em>Escherichia coli</em> Inducible Lysine Decarboxyase","year":2010,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; National Institutes of Health; University of Toronto","keywords":"Lysine decarboxylase; Decarboxylation; Biochemistry; Escherichia coli; Lysine; Ornithine decarboxylase; Cadaverine; Arginine decarboxylase; Antiporter; Carboxy-lyases; Amino acid; Ornithine decarboxylase antizyme; Substrate (aquarium); Biology; Enzyme; Chemistry; Spermine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001231472,0.002893016,0.001343974,0.002244032,0.0004119302,0.001000767,0.001388029,0.002077322,0.00254558],"category_scores_gemma":[0.001535595,0.001004993,0.0009695662,0.002234232,0.0006278122,0.0007055225,0.001212528,0.003143064,0.003368067],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005656548,"about_ca_system_score_gemma":0.0006336721,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007259913,"about_ca_topic_score_gemma":0.001236364,"domain_scores_codex":[0.996051,0.0009187569,0.0004361361,0.0007388632,0.001566563,0.0002887936],"domain_scores_gemma":[0.9979886,0.000383216,0.0004409597,0.0002535159,0.0005705354,0.0003631697],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001431566,0.0003299139,0.001617924,0.0002455362,0.00004018158,0.00005701159,0.00004632835,0.0001010346,0.991326,0.0001784975,0.0005177869,0.005396658],"study_design_scores_gemma":[0.00003964482,0.0007309038,0.006867221,0.00007968274,0.00006314216,0.0003439393,0.00005023519,0.002684872,0.9824738,0.0001558182,0.006481377,0.00002940646],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4419064,0.01342896,0.4901716,0.001063494,0.001613143,0.002550433,0.01332902,0.009303028,0.02663382],"genre_scores_gemma":[0.4551361,0.009691401,0.4565029,0.0007827369,0.0002586125,0.003660293,0.02698027,0.0008309626,0.04615672],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002893016,"threshold_uncertainty_score":0.008515775,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02484434863020415,"score_gpt":0.3618115335649344,"score_spread":0.3369671849347303,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}