{"id":"W2048309218","doi":"10.1016/j.mimet.2004.02.002","title":"Designing better probes: effect of probe size, mismatch position and number on hybridization in DNA oligonucleotide microarrays","year":2004,"lang":"en","type":"article","venue":"Journal of Microbiological Methods","topic":"Gene expression and cancer classification","field":"Biochemistry, Genetics and Molecular Biology","cited_by":143,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"DNA microarray; Amplicon; Oligonucleotide; Hybridization probe; Biology; Oligomer restriction; DNA; Computational biology; Base pair; Microarray; Nucleic acid thermodynamics; GC-content; Genetics; Molecular biology; Gene; Biological system; Gene expression; Base sequence; Polymerase chain reaction; Genome","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.006712871,0.001496869,0.00176368,0.0006666677,0.0005120575,0.001298091,0.001025266,0.002337417,0.001849268],"category_scores_gemma":[0.01293584,0.003025664,0.000756088,0.0007296111,0.0008733914,0.002739054,0.0006976487,0.001849283,0.0007725304],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005648534,"about_ca_system_score_gemma":0.0004607252,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002337263,"about_ca_topic_score_gemma":0.001219272,"domain_scores_codex":[0.9944922,0.00293225,0.0005512658,0.000913667,0.0009079052,0.0002027241],"domain_scores_gemma":[0.988925,0.009187616,0.0006017383,0.0005195772,0.0005614868,0.0002045381],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001787645,0.00005997526,0.0002081406,0.0001715618,0.00001914221,0.00002894086,0.00005906864,0.0007776316,0.9921728,0.0001340554,0.00009706226,0.006092883],"study_design_scores_gemma":[0.00002666397,0.0003021123,0.0005619564,0.00001723016,0.00006445617,0.0001298327,0.00001676499,0.004538767,0.9931929,0.0001417485,0.0009813843,0.00002634009],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6713816,0.009878118,0.311496,0.001831025,0.0005319726,0.0005094629,0.0002812273,0.002613581,0.001477006],"genre_scores_gemma":[0.4898716,0.004141109,0.4994279,0.001501578,0.0001492225,0.0006856332,0.0004491579,0.001035979,0.002737877],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006712871,"threshold_uncertainty_score":0.03550148,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0113894461644135,"score_gpt":0.3160029310965136,"score_spread":0.3046134849321001,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}