{"id":"W2049341916","doi":"10.1006/prep.2001.1576","title":"Expression and Refolding of Recombinant Human α-Tocopherol Transfer Protein Capable of Specific α-Tocopherol Binding","year":2002,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Antioxidant Activity and Oxidative Stress","field":"Medicine","cited_by":20,"is_retracted":false,"has_abstract":false,"ca_institutions":"Brock University","funders":"","keywords":"Recombinant DNA; Fusion protein; Chemistry; Biochemistry; FLAG-tag; Affinity chromatography; Escherichia coli; Amino acid; Histidine; Protein A/G; Expression vector; Protein G; Inclusion bodies; Molecular biology; Biology; Enzyme; Antibody; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000336661,0.0002209236,0.0004424534,0.0001858872,0.0002161138,0.00002178298,0.00009447294,0.0001941803,0.0004996599],"category_scores_gemma":[0.00004689543,0.0001785422,0.00006888469,0.0001992202,0.0001911019,0.0003118569,0.0000386978,0.0002445737,0.000006463303],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002426473,"about_ca_system_score_gemma":0.00001159464,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002588314,"about_ca_topic_score_gemma":0.000001116688,"domain_scores_codex":[0.9983097,0.0001691348,0.0005316332,0.0004381786,0.0003283556,0.0002230381],"domain_scores_gemma":[0.9991041,0.00003785879,0.0002612682,0.0003500363,0.0001164518,0.0001302482],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002430593,0.0003518551,0.0009043526,0.0006803562,0.00001194494,0.000002427848,0.0009054868,5.884854e-7,0.9875114,0.0005508268,0.0001253743,0.008712318],"study_design_scores_gemma":[0.001962888,0.0003795696,0.002402898,0.003074436,0.00002131795,0.000007711792,0.0003818794,0.0001139938,0.9899893,0.0002135804,0.001274814,0.0001775691],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9932584,0.001158109,0.001569881,0.0001862315,0.00003396079,0.00273157,0.00001069277,0.00004084555,0.00101038],"genre_scores_gemma":[0.9949823,0.0003180963,0.002842761,0.00000596042,0.00004487905,0.000318516,0.00002188615,0.00002847988,0.001437143],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008534749,"threshold_uncertainty_score":0.7280738,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0411029797622165,"score_gpt":0.2660038125623498,"score_spread":0.2249008328001333,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}