{"id":"W2049965804","doi":"10.1016/j.mimet.2003.07.003","title":"New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica","year":2003,"lang":"en","type":"article","venue":"Journal of Microbiological Methods","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":281,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Institut national de la recherche scientifique","keywords":"Yarrowia; Biology; Hygromycin B; Recombinase; Gene; Gene knockout; Marker gene; Auxotrophy; Mutant; Gene targeting; Locus (genetics); Genetics; Yeast; Cre recombinase; Cre-Lox recombination; Selectable marker; Recombination; Plasmid; Transgene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000662347,0.0008316062,0.0008969428,0.0006604597,0.0004139184,0.0007981007,0.001058942,0.0009179002,0.001665978],"category_scores_gemma":[0.0005115326,0.0008435127,0.0009217291,0.0004755537,0.0005403757,0.0006925397,0.0009927748,0.003529013,0.001669302],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006417047,"about_ca_system_score_gemma":0.0003700504,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005334002,"about_ca_topic_score_gemma":0.0008376433,"domain_scores_codex":[0.9994928,0.00004283422,0.0001021235,0.0001008295,0.0001947641,0.00006661149],"domain_scores_gemma":[0.9994324,0.0001565696,0.0001637388,0.0001133828,0.00004196143,0.00009200389],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006653976,0.00003375025,0.00006351177,0.00005167658,0.000006658334,0.00006426692,0.00002972495,0.00006413026,0.9962604,0.000277087,0.000095986,0.002986359],"study_design_scores_gemma":[0.0000830107,0.0001480014,0.002464772,0.00002609665,0.00006781406,0.0007603305,0.00003356905,0.00109975,0.9845328,0.0001723707,0.01056953,0.00004207291],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7493442,0.004599905,0.2296816,0.001406564,0.0004977031,0.0008627517,0.004320861,0.006224227,0.003062229],"genre_scores_gemma":[0.8591879,0.004248973,0.1085562,0.0003838161,0.000156466,0.0009399781,0.01082235,0.002529411,0.01317492],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001665978,"threshold_uncertainty_score":0.005573273,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03915667770779752,"score_gpt":0.3731487011023387,"score_spread":0.3339920233945412,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}