{"id":"W2050207946","doi":"10.1371/journal.pone.0009225","title":"Visualizing Interactions along the Escherichia coli Twin-Arginine Translocation Pathway Using Protein Fragment Complementation","year":2010,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Division of Chemical, Bioengineering, Environmental, and Transport Systems; Canadian Institutes of Health Research; New York State Foundation for Science, Technology and Innovation; National Science Foundation","keywords":"Bimolecular fluorescence complementation; Twin-arginine translocation pathway; Translocase; Complementation; Biology; Chaperone (clinical); Cell biology; Transmembrane domain; Protein-fragment complementation assay; Cytoplasm; Protein–protein interaction; Transport protein; Transmembrane protein; Biochemistry; Yellow fluorescent protein; Escherichia coli; Membrane protein; Biophysics; Chromosomal translocation; Membrane transport protein; Membrane; Mutant; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001479348,0.0001156603,0.0001050715,0.00003186509,0.0001653341,0.00004072801,0.0001475846,0.0001189621,0.00007702391],"category_scores_gemma":[0.00002862852,0.00009748206,0.000038042,0.00009381164,0.00007137435,0.000004887419,0.00005725594,0.0001863672,0.000006476763],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001159373,"about_ca_system_score_gemma":0.0000498568,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008334982,"about_ca_topic_score_gemma":0.0005406313,"domain_scores_codex":[0.9992239,0.00004718585,0.0002191861,0.0002218907,0.0001167847,0.0001710498],"domain_scores_gemma":[0.9994984,0.000006186664,0.0001055502,0.0002636159,0.00009058467,0.00003561295],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002879775,0.0003879024,0.0002434677,0.00001429973,0.0000889824,2.891775e-7,0.00006868021,0.000004288131,0.9976655,0.0002335633,0.00001224349,0.001252016],"study_design_scores_gemma":[0.0003668899,0.0001528372,0.0008881012,0.00003150597,0.00003676019,0.00000160144,0.0001010872,0.0006651136,0.994293,0.00003604631,0.003301388,0.0001256931],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965637,0.00006082626,0.001766044,0.0008279247,0.0001339632,0.0005404288,0.00001852125,0.00002014225,0.00006843438],"genre_scores_gemma":[0.9894564,0.00002720779,0.009690815,0.0001575746,0.0003555571,0.00007755557,0.0001491115,0.00001936346,0.00006640763],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007924771,"threshold_uncertainty_score":0.3975203,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0388897198727938,"score_gpt":0.2767683404872689,"score_spread":0.2378786206144751,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}