{"id":"W2050207946","doi":"10.1371/journal.pone.0009225","title":"Visualizing Interactions along the Escherichia coli Twin-Arginine Translocation Pathway Using Protein Fragment Complementation","year":2010,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Division of Chemical, Bioengineering, Environmental, and Transport Systems; Canadian Institutes of Health Research; New York State Foundation for Science, Technology and Innovation; National Science Foundation","keywords":"Bimolecular fluorescence complementation; Twin-arginine translocation pathway; Translocase; Complementation; Biology; Chaperone (clinical); Cell biology; Transmembrane domain; Protein-fragment complementation assay; Cytoplasm; Protein–protein interaction; Transport protein; Transmembrane protein; Biochemistry; Yellow fluorescent protein; Escherichia coli; Membrane protein; Biophysics; Chromosomal translocation; Membrane transport protein; Membrane; Mutant; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006362079,0.0006451765,0.0004088318,0.0007685393,0.0004537035,0.0006308285,0.0008177726,0.0009033469,0.001117381],"category_scores_gemma":[0.0002872657,0.0002326904,0.000335847,0.0007020981,0.0006430317,0.0004572296,0.0008079789,0.001502987,0.0004322298],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009000319,"about_ca_system_score_gemma":0.000499606,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002079574,"about_ca_topic_score_gemma":0.001186643,"domain_scores_codex":[0.9993755,0.000128183,0.00003684197,0.0001347609,0.0002151258,0.0001096191],"domain_scores_gemma":[0.9995684,0.0001468214,0.00009730445,0.00005713358,0.00006561345,0.00006468522],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003883263,0.00002571465,0.0001083947,0.00003088096,0.000004192089,0.00006049936,0.0000269802,0.0001916224,0.9977876,0.000605458,0.00004717819,0.001072702],"study_design_scores_gemma":[0.00001162196,0.00005433051,0.0008246735,0.00000524964,0.000005237039,0.0002384959,0.00003787578,0.004434593,0.9924616,0.0001837946,0.00172998,0.0000126258],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9081947,0.000760924,0.08542075,0.0003895299,0.00004518498,0.0001330984,0.001232937,0.000611521,0.003211413],"genre_scores_gemma":[0.9114744,0.001042204,0.08348153,0.0001354107,0.000008288322,0.0002281118,0.001232338,0.0001421943,0.002255553],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002079574,"threshold_uncertainty_score":0.006530225,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0388897198727938,"score_gpt":0.2767683404872689,"score_spread":0.2378786206144751,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}