{"id":"W2051193695","doi":"10.1074/jbc.m108744200","title":"Mapping Cu(II) Binding Sites in Prion Proteins by Diethyl Pyrocarbonate Modification and Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometric Footprinting","year":2002,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":141,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Occupational Cancer Research Centre","funders":"","keywords":"Chemistry; Histidine; Footprinting; Copper; Binding site; Protease; Peptide; Metalloprotein; Desorption; Mass spectrometry; Molecule; Biochemistry; Stereochemistry; Enzyme; Chromatography; DNA; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001989815,0.0003466622,0.0001860446,0.0003367781,0.0002123809,0.0002999565,0.0003203752,0.0002924479,0.0004712916],"category_scores_gemma":[0.0003958185,0.0002224471,0.0001539061,0.0002144148,0.0002959926,0.0001954604,0.0002219937,0.0003809696,0.0002631525],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002347304,"about_ca_system_score_gemma":0.0001481236,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006232012,"about_ca_topic_score_gemma":0.001076115,"domain_scores_codex":[0.9998281,0.00002969309,0.000009340611,0.00004257848,0.00007035969,0.00001999554],"domain_scores_gemma":[0.9997626,0.00005629078,0.00008604775,0.00002364644,0.00003617168,0.00003514507],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003625738,0.000006251186,0.0001616345,0.00001773516,0.00000224762,0.00002373782,0.000009845101,0.00002740839,0.9986727,0.00002263044,0.000009487085,0.001010027],"study_design_scores_gemma":[0.000009013383,0.00009561497,0.006191303,0.000003142128,0.00001072133,0.0005967257,0.00001825787,0.0009497894,0.9911079,0.00007251729,0.0009369879,0.000008043241],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9747902,0.001401952,0.02266103,0.00008337948,0.00001171216,0.00003780823,0.0002606639,0.0001007829,0.0006523447],"genre_scores_gemma":[0.9313416,0.001389998,0.06436119,0.00008759578,0.0000171374,0.00006811176,0.0007204925,0.00003962556,0.001974285],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006232012,"threshold_uncertainty_score":0.001703024,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02060845230042121,"score_gpt":0.2412250088218435,"score_spread":0.2206165565214223,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}