{"id":"W2053001938","doi":"10.1371/journal.pone.0042791","title":"Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells","year":2012,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":52,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Institute of Neurological Disorders and Stroke; National Institute of Mental Health; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Alberta Innovates","keywords":"Diacylglycerol kinase; Second messenger system; Intracellular; Inositol; Phospholipase C; Cell biology; Phospholipase; Phosphoinositide phospholipase C; Fluorescence; Calcium; Biology; Biophysics; Biochemistry; Chemistry; Signal transduction; Protein kinase C; Receptor; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004557847,0.000469245,0.0006791975,0.0002758324,0.0002682203,0.0006622719,0.0008081802,0.001069136,0.000768427],"category_scores_gemma":[0.0004208155,0.0003465854,0.0002353554,0.0003618611,0.0006619826,0.0006991316,0.0007043369,0.0008487033,0.0006720469],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006154029,"about_ca_system_score_gemma":0.0003247983,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005941972,"about_ca_topic_score_gemma":0.0009580704,"domain_scores_codex":[0.9993303,0.00007394588,0.00003574869,0.0002660035,0.0002156929,0.00007826526],"domain_scores_gemma":[0.9997136,0.00008627697,0.0000452816,0.00004469094,0.00006066321,0.00004945812],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002638142,0.000005479099,0.00004806235,0.00001890292,0.000002732837,0.00001751656,0.000007098532,0.00002858857,0.9986171,0.0001368944,0.00003430052,0.001056934],"study_design_scores_gemma":[0.00001122431,0.00004733082,0.0004738793,0.000003270919,0.000008332257,0.0001083472,0.00001038036,0.001126831,0.9963164,0.000133187,0.00175197,0.000008808671],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8135505,0.006771529,0.1706879,0.0008254888,0.000318448,0.0002139541,0.001047664,0.001115228,0.005469373],"genre_scores_gemma":[0.8037649,0.004033163,0.1851577,0.0005011931,0.00008602069,0.0004045403,0.0008101379,0.0001048736,0.005137405],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001069136,"threshold_uncertainty_score":0.004465103,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01426395175350436,"score_gpt":0.2021198113353297,"score_spread":0.1878558595818254,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}