{"id":"W2053629505","doi":"10.4155/bio.10.74","title":"Assessing Covalent Binding of Reactive Drug Metabolites by Complete Protein Digestion and LC–MS Analysis","year":2010,"lang":"en","type":"article","venue":"Bioanalysis","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université du Québec à Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Cysteine; Biochemistry; Amino acid; Drug; Covalent bond; Microsome; Drug metabolism; Digestion (alchemy); Chromatography; In vitro; Metabolism; Enzyme; Pharmacology; Biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008106225,0.0008554412,0.0004902156,0.0006014363,0.0002539892,0.0003131059,0.0004387441,0.0005460925,0.00119664],"category_scores_gemma":[0.0009055919,0.0002364406,0.0003537703,0.0003858333,0.0004275768,0.0003382412,0.0002756092,0.0005388519,0.0005776808],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003819948,"about_ca_system_score_gemma":0.000475388,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004375052,"about_ca_topic_score_gemma":0.0008522548,"domain_scores_codex":[0.9992926,0.0001262628,0.00005519075,0.0001511477,0.0003152955,0.00005942016],"domain_scores_gemma":[0.9992445,0.0001993921,0.0002212381,0.00008224899,0.0001993993,0.00005330054],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004840839,0.00001611799,0.0003351155,0.00004742675,0.00001650004,0.00001836748,0.000009667026,0.00005788368,0.9975991,0.00002969175,0.00001805181,0.001803725],"study_design_scores_gemma":[0.000003362016,0.0001655808,0.001462176,0.000002832122,0.00002177875,0.0001072326,0.000005552127,0.0005113654,0.9971796,0.00002517055,0.0005110661,0.000004382421],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.830888,0.005547542,0.1590176,0.0001432851,0.00006768251,0.0003593349,0.0009452728,0.000482698,0.002548631],"genre_scores_gemma":[0.8422875,0.004490454,0.1475268,0.0001949027,0.00004216595,0.0004232725,0.001490881,0.0001097653,0.003434111],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00119664,"threshold_uncertainty_score":0.004287064,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01043854655386458,"score_gpt":0.2618184679784494,"score_spread":0.2513799214245849,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}