{"id":"W2053629505","doi":"10.4155/bio.10.74","title":"Assessing Covalent Binding of Reactive Drug Metabolites by Complete Protein Digestion and LC–MS Analysis","year":2010,"lang":"en","type":"article","venue":"Bioanalysis","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université du Québec à Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Cysteine; Biochemistry; Amino acid; Drug; Covalent bond; Microsome; Drug metabolism; Digestion (alchemy); Chromatography; In vitro; Metabolism; Enzyme; Pharmacology; Biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003791111,0.0001521156,0.0003423251,0.0002542518,0.00009900875,0.00007465517,0.000112948,0.0001028242,0.00003180769],"category_scores_gemma":[0.0001492723,0.000134545,0.0002369366,0.0007909031,0.0001231884,0.00002217215,0.00006593711,0.00008503992,0.000001310016],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009389354,"about_ca_system_score_gemma":0.00002270948,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001797112,"about_ca_topic_score_gemma":0.0001400487,"domain_scores_codex":[0.9988681,0.00008729992,0.0003172484,0.0004020869,0.0001767294,0.0001485501],"domain_scores_gemma":[0.9990665,0.00001923551,0.0003014437,0.0003634922,0.0001809128,0.00006846314],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000008574901,0.00005573928,0.0238513,0.00001313901,0.000987184,1.822223e-7,0.00003136635,0.00002334264,0.9737708,0.000046232,0.00001974457,0.001192412],"study_design_scores_gemma":[0.0001818521,0.00002517584,0.01262243,0.000006080734,0.002129016,7.646988e-7,0.00017466,0.000995578,0.9808951,0.00001135499,0.00277555,0.0001824172],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9948471,0.00142487,0.003298535,0.0000589878,0.0000255209,0.0001170039,0.00008184193,0.000007830182,0.0001383425],"genre_scores_gemma":[0.9966094,0.0001052716,0.001487695,0.00001380367,0.00004000632,0.00001777554,0.001396497,0.00001245681,0.0003170774],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01122888,"threshold_uncertainty_score":0.5486585,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01043854655386458,"score_gpt":0.2618184679784494,"score_spread":0.2513799214245849,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}