{"id":"W2053911700","doi":"10.1074/jbc.m707941200","title":"Bioluminescence Resonance Energy Transfer Assays Reveal Ligand-specific Conformational Changes within Preformed Signaling Complexes Containing δ-Opioid Receptors and Heterotrimeric G Proteins","year":2008,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":109,"is_retracted":false,"has_abstract":true,"ca_institutions":"Montreal Clinical Research Institute; Université de Montréal; Institut universitaire en santé mentale de Montréal","funders":"National Institute on Drug Abuse","keywords":"Heterotrimeric G protein; Bioluminescence; Chemistry; Förster resonance energy transfer; G protein-coupled receptor; Energy transfer; Receptor; Cell biology; G protein; Biophysics; Ligand (biochemistry); Biochemistry; Biology; Physics; Fluorescence","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000281375,0.0003428991,0.0001851933,0.0002042935,0.000120075,0.0002985018,0.0002999971,0.0003419479,0.0007470588],"category_scores_gemma":[0.0002740575,0.0001531261,0.0001475446,0.0001420055,0.0003197323,0.0003025436,0.0002125205,0.0006880813,0.000218484],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003222533,"about_ca_system_score_gemma":0.00008346204,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001998446,"about_ca_topic_score_gemma":0.0002575974,"domain_scores_codex":[0.999793,0.00004034631,0.000009909329,0.00006228782,0.00004735315,0.00004720462],"domain_scores_gemma":[0.9998055,0.00007716147,0.00005288594,0.00002253013,0.00001555158,0.00002641783],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004392086,0.00001193719,0.0001158923,0.000009694341,0.000002484385,0.00001397569,0.00001381554,0.00003381909,0.9991611,0.00009968433,0.000008840309,0.0004847455],"study_design_scores_gemma":[0.000008297571,0.0001129854,0.002021817,0.000002027399,0.000005194206,0.0001120824,0.00002438948,0.0009708971,0.996435,0.00007976718,0.0002238163,0.000003662899],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9904593,0.0004795697,0.00820647,0.00005226909,0.000008413435,0.0000101391,0.00006315731,0.00005191147,0.000668816],"genre_scores_gemma":[0.9941505,0.0002208726,0.004413083,0.0000545957,0.00000436561,0.00002228958,0.0001460873,0.000009846397,0.0009783347],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007470588,"threshold_uncertainty_score":0.002499163,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02898053875183498,"score_gpt":0.226611963430311,"score_spread":0.197631424678476,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}