{"id":"W2053911955","doi":"10.2478/s11756-010-0017-7","title":"Codon optimization, expression and characterization of Bacillus subtilis MA139 β-1,3-1,4-glucanase in Pichia pastoris","year":2010,"lang":"en","type":"article","venue":"Biologia","topic":"Biofuel production and bioconversion","field":"Engineering","cited_by":7,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"University of Saskatchewan","keywords":"Glucanase; Pichia pastoris; Bacillus subtilis; Brewing; Alcohol oxidase; Recombinant DNA; Fermentation; Pichia; Biochemistry; Chemistry; Biology; Food science; Microbiology; Bacteria; Enzyme; Gene; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002319112,0.0004214451,0.0003265192,0.0002273391,0.0001703738,0.0003628973,0.0002345977,0.0001720289,0.0003456975],"category_scores_gemma":[0.0002836599,0.000178705,0.0002575446,0.0005003511,0.0001315281,0.0002062197,0.0001923252,0.0004245451,0.000233749],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003467423,"about_ca_system_score_gemma":0.0002313884,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002297389,"about_ca_topic_score_gemma":0.002172301,"domain_scores_codex":[0.9997895,0.00003926853,0.00003819352,0.00002862246,0.00007316813,0.00003128886],"domain_scores_gemma":[0.9999281,0.00001569154,0.00001527608,0.00001391714,0.00001630775,0.00001063289],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007282951,0.00003153952,0.000193351,0.00002627404,0.000005839484,0.00003549497,0.00001941595,0.0002352501,0.9978224,0.00005301987,0.00003416065,0.001470411],"study_design_scores_gemma":[0.000006318962,0.00006505757,0.002568735,0.000003135371,0.00001152562,0.00006359568,0.0000166885,0.0008026974,0.9952381,0.00002198135,0.001196479,0.000005700954],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9931288,0.0006787553,0.004121494,0.0001094097,0.00002739036,0.00003272203,0.0008409452,0.00006903662,0.0009915406],"genre_scores_gemma":[0.9823015,0.0009844133,0.008516483,0.00005806161,0.00001161773,0.0000496028,0.00346101,0.0001032497,0.004514066],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002297389,"threshold_uncertainty_score":0.00456804,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006532141567429347,"score_gpt":0.1818344174400873,"score_spread":0.1753022758726579,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}